雷公藤多苷干预TLR-NF-κB通路发挥免疫抑制作用.PDFVIP

·1288 · 中草药 Chinese Traditional and Herbal Drugs 第 45 卷 第 9 期 2014 年 5 月 雷公藤多苷干预 TLR-NF-κB 通路发挥免疫抑制作用 * 张 敏 ，王守安，刘黎星 莆田学院基础医学院 生理学教研室，福建 莆田 351100 摘 要：目的 探讨雷公藤多苷在变应性鼻炎（allergic rhinitis，AR ）发病中干预 TLR-NF-κB 通路的机制。方法 100 只大鼠随机分成 4 组：对照组、模型组、雷公藤多苷组、倍氯米松组，每组 25 只。应用卵清白蛋白制备 AR 模型，施加 雷公藤多苷干预。HE 染色观察鼻黏膜形态改变并计数炎性细胞浸润数，免疫组化法检测肿瘤坏死因子-α （TNF-α）、白细 胞介素-5（IL-5 ）及免疫球蛋白（IgE ）的量，实时定量 PCR 及 Western blotting 检测 Toll 样受体（TLR4 ）和核因子-κB （NF-κB ） 的表达情况。结果 模型组变应性损伤明显，鼻黏膜以嗜酸性粒细胞浸润为主，TNF-α、IL-5 及 IgE 的表达较对照组明显 增高，TLR4 和 NF-κB 的表达较对照组也明显增高（P ＜0.05 ）；雷公藤多苷及倍氯米松组鼻黏膜以少量中性粒细胞浸润为 主，IL-5 、IgE 、TLR4 和 NF-κB 的表达较模型组明显降低（P ＜0.05 ）。结论 雷公藤多苷可通过影响 TLR-NF-κB 信号传 导通路，降低 TLR4 及 NF-κB 的表达发挥免疫调节作用。 关键词：雷公藤多苷；Toll 样受体；核因子-κB ；TLR-NF-κB 通路；变应性鼻炎 中图分类号：R765.21 文献标志码：A 文章编号：0253 - 2670(2014)09 - 1288 - 05 DOI: 10.7501/j.issn.0253-2670.2014.09.017 Immunosuppression of tripterygium glycosides via TLR-NF-κB signaling pathway ZHANG Min, WANG Shou-an, LIU Li-xing Department of Physiology of Basic Medical College, Putian University, Putian 351100, China Abstract: Objective To investigate the intervention of tripterygium glycosides (TG) on TLR-NF-κB signaling pathway in pathogenesis of allergic rhinitis (AR). Methods Ovalbumin was provided to build AR model, TG was used to interfere the rats, and 100 rats were randomly divided into four groups such as control (group A), model (group B), TG (group C), BDP (group D) groups (n = 25). Changes of nasal mucosa tissues and inflammatory cell infiltration were observed by HE staining, while neutrophil and eosinophil count was performed under high power microscope. Expression of IL-5, TNF-α, and IgE in nasal mucosa tissues was measured with immunohistochemical method. Realtime-PCR and Western-blotting were used to evaluate the expression levels of TLR4 and NF-κB. Results Developed AR injury of nasal