骨髓基质细胞对移植肌腱在骨隧道内愈合作用的影响-信息安全研究.PDFVIP

天津医药 20 12 年 9 月第 40 卷第 9 期 923 doi:10.3969/j.i ssn.0253-9896.2012.09.018 骨髓基质细胞对移植肌腱在骨隧道内愈合作用的影响* 曹红彬 辛景义 梁 军 李秀兰 于顺禄 摘要 目的 研究骨髓基质细胞对移植肌腱在骨隧道内愈合方式的影响 方法 采用兔膝关节自体半腱肌重建 前交叉韧带悬吊固定模型袁实验分为空白对照组尧胶原海绵组和骨髓基质细胞渊MSCs 冤组袁MSCs 组在骨隧道内植入骨 髓基质细胞 分别于手术后4尧8 和12 周采取组织袁采用 HE尧Masson 染色袁观察骨隧道和肌腱移植物 的界面组织学 特殊变化袁分析腱骨愈合信息 结果 4 周时MSCs 组腱骨间充满结缔组织袁空白对照组和胶原海绵组腱骨间有少量 结缔组织曰8 周 MSCs 组形成Sharpey 爷s 纤维袁空白对照组合胶原海绵组无Sharpey 爷s 纤维出现曰12 周时MSCs 组形成 大量Sharpey 爷s 纤维袁在移植肌腱和骨隧道之 有纤维软骨袁矿化软骨形成袁空白对照组和胶原海绵组出现Sharpey 爷s 纤维袁无矿化软骨形成 结论 MSCs 对移植肌腱在骨隧道内的愈合起明显的促进作用袁可以缩短腱骨愈合时 关键词 骨髓细胞 肌袁 骨骼 腱 骨-髌韧带-骨组织移植重建术 腱转移术 前交叉韧带 组织学技术 骨髓基质细胞 骨隧道 The Histological Study of Mesenchymal Stem Cells on Tendon-Bone Healing in Bone Tunnel CAO Hongbin袁XIN J ingyi 袁LIANG Jun 袁LI Xiulan 袁YU Shunlu Departme nt of Traumatic Orthopaedics , Tianj in Hospital, Tianj in 300211, China Abstract Obj ective: To investigate the effect of mesenchymal stem cells (MSCs) on tendon-bone healing in bone tunnel. Methods: Anterior cruciate ligament reconstruction with auto-semitendinous tendon was established in knee j oints of New Zealand rabbits by suspensory fixation model. All animals were divided into blank group, collagen sponge group and MSCs group. MSCs were implanted in bone tunnel in MSCs group. The collagen sponge was implanted in bone tunnel in collagen sponge group and only operation in blank group. Specimens were collected 4 weeks, 8 weeks and 12 weeks after surgery . Sections were stained with HE and Masson. Special changes of interface tissue between bone tunnel and tendon graft were observed and analyzed. Results: After 4 weeks, an abundant amount of connective tissues was observed in MSC