转基因豆粕调控元件在饲料加工中降解变化的初步研究-华北农学报.pdfVIP

# 2009, 24 ( 2) : 2012 205 1 2 1 3 梁 杉 , 梁 宁, 蒋继志, 胡东升 ( 1. , 071002; 2. , 071002;3. , 075000) : , CaMV35S NOS , PCR , ; , 246, 165 101 bp 3 , 125 bp , 217 bp ;, , :; ; ; :Q78 :A : 1000- 7091( 2009)02- 0201- 05 Degradation of Non- target Genes During Feed Processes of Genetically Modified Soybean Meal 1 2 1 3 LIANG Sha ,LIANG Ni g , JIANG Ji- zhi ,HU Do g- she g ( 1. College of Life Scie ces, Hebei U iversity, Baodi g 071002, Chi a;2. Library of Hebei U iversity, Baodi g 071002, Chi a; 3. I ovatio High School of Hebei, Zha gjiakou 075000, Chi a) Abstract:I order to privide some k owledge for tra sge ic pla t feed, primeres were desig ed o tra sge ic soy2 bea meal withi CaMV35 Spromoter ge e a d NOS termi ator, a d effect of heat treatme t, autoclave, a d other process2 i g tech ics o degradatio of CaMV35 S a d NOS i soybea meal was i vestigated by polymerase chai reactio detec2 tio system i this experime t. The results showed that heat treatme t had o effect o degradatio of both CaMV35 S a d N OS.Autoclave treatme t also did ot affect degradatio of CaMV35 S, a d 246, 165, a d 101bp fragme ts of CaMV35 S were all detectable, but resulted i degradatio of NOS , a d 125bp fragme t was detectable a d 217 bp fragme t was u 2 detectable.A d the same result was obtai ed also i feeds those processed from soybea meal.The promoter ge e was rel2 atively stable a d termi ator ge e was relatively easily destroyed. Key words:Soybea meal;Promoter;Termi ator;Feed processi g , CaMV 3 Kohli[2] 35S , Cummi s[2] , , :