马铃薯甲虫两个系统性rna干扰缺失基因cdna的克隆-应用昆虫学报.pdfVIP

Chinese Journal of Applied Entomology 2016, 53(2): 347356. DOI: 10.7679/j.issn.20951353.2016.046 马铃薯甲虫两个系统性 RNA 干扰缺失基因 cDNA 的克隆、序列分析和时空表达* 1** 2 3 4 2 2*** 杨 帅 付开赟 吴家和 李国清 吐尔逊 郭文超 1. 832003 2. 830091 3. 100101; 4. 210095 摘 要 【目的】 Leptinotarsa decemlineata (Say) Sid-1 【方法】 RT-PCR 5/3 RACE qPCR 【结果】 Leptinotarsa decemlineata 4 LdeSid-1a LdeSid-1cmRNA 2 887 3 733 bp759 782 86.19 90.27 ku 59% RNA LdeSid-1a LdeSid-1c RNA 11 N 4 qPCR LdeSid-1a LdeSid-1c LdeSid-1c LdeSid-1a LdeSid-1c GenBank KR153284 KR153285【结论】 LdeSid-1a LdeSid-1c RNA 关键词 RNA PCRmRNA Molecular cloning, sequence analysis and expression profiles of LdeSid-1a and LdeSid-1c genes in Leptinotarsa decemlineata (Say) 1** 2 3 4 YANG Shuai FU Kai-Yun WU Jia-He LI Guo-Qing 2 2*** Tuerxun GUO Wen-Chao (1. College of Agronomy, Shihezhi University, Shihezi 832003, China; 2. Research Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 3. State Key Laboratory of Plant Genomics, Institute of Microbiology, Chinese Academy of Science; 4. Department of Entomology, College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China) Abstract [Objectives] To clone and analyze two Sid-1 full coding sequences and expression profiles in Leptinotarsa decemlineata. [Methods] Full coding sequences of two Sid-1s were amplified by RT-PCR and RACE-PCR, conserved motifs and origin of