干旱对水稻生物钟基因和干旱胁迫响应基因每日节律性变化的-遗传.pdfVIP

干旱对水稻生物钟基因和干旱胁迫响应基因每日节 律性变化的影响 李佳，刘运华，张余，陈晨，余霞，余舜武 上海市农业生物基因中心，上海201106 摘要：内源生物钟的节律运动不仅调控植物的生长发育，而且在调控植物响应和适应环境过程中发挥重要 的作用。为了解水稻(Oryza sativa L.)干旱胁迫响应基因和生物钟基因在干旱条件下每日表达变化情况，本 文利用实时荧光定量PCR 方法研究旱稻品种IRAT109 在干旱胁迫下相关基因的表达变化。结果表明，干旱 胁迫导致早晨生物钟基因OsPRRs、OsLHY 和OsZTL1 的表达量显著下降，振幅减弱；同时导致夜晚生物钟 基因OsTOC1、OsGI 和OsELF3 整体表达量升高，振幅增强，但对OsFKF1 基因影响不大。同样，大部分 水稻干旱胁迫响应基因在干旱胁迫后整体表达量显著升高，但OsDST 基因表达量下降；同时大部分抗逆基 因周期性表达被扰乱，但OsCIPK12、OsCDPK7 和OsDREB1A 依然保持24 h 内震荡。本研究结果表明干 旱胁迫能影响生物钟元件的基因表达，这种互相影响改变了部分基因每日的震荡变化。 关键词：干旱；水稻；生物钟基因；胁迫响应基因 Drought stress modulates diurnal oscillations of circadian clock and drought-responsive genes in Oryza sativa L. Jia Li, Yunhua Liu, Yu Zhang, ChenChen, Xia Yu, Shunwu Yu Shanghai Agrobiological Gene Center, Shanghai 201106, China Abstract: Endogenous circadian rhythms play a key role in regulating plant growth and development, and in allowing plants to respond and adapt to changing environments. To understand how drought regulates upland rice(Oryza sativa L.) IRAT109, we examined circadian clock and drought-responsive genes through real-time PCR. The results revealed that, first, drought reduced the relative expression level and amplitude of peak expression of several morning circadian clock components (such as OsPRRs, OsLHY and OsZTL1), increased the relative expression level and amplitude of some evening circadian clock components (such as OsTOC1, OsGI and OsELF3), but did not influence OsFKF1. Secondly, the relative expression level of most drought-responsive genes was generally increased, except for OsDST, a negative regulator. Lastly, expression rhythms of most drought-responsive genes were disturbed, but not that of OsCIPK12, OsCDPK7 and OsDREB1A. The results indicate that drought stress modulates the expression of circadian clock