强化阿托伐他汀对不稳定型心绞痛患者PCI围术期CD4+T淋巴细胞 Sprouty1表达影响.docVIP

强化阿托伐他汀对不稳定型心绞痛患者PCI围术期CD4+T淋巴细胞 Sprouty1表达影响 　　DOI：10.3760/cma.j.issn.1671-0282.2013.08.021 基金项目：国家自然科学基金资助项目 作者单位：530021南宁，广西医科大学第一附属医院心内科广西心血管病研究所 通信作者：李浪，Email：drlilang@163.com 【摘要】目的探讨强化剂量他托伐他汀对不稳定心绞痛患者PCI围术期CD4+T淋巴细胞Sprouty1表达的影响。方法入选广西医科大学第一附属医院住院不稳定型心绞痛患者52例，随机（随机数字法）分为强化他汀组（术前80 mg/d，术后40 mg/d，n=26）和常规他汀组（术前术后均20 mg/d，n=26）。分别于PCI术前、术后16～24 h抽取新鲜外周血，免疫磁珠法分选出CD4+T淋巴细胞，荧光定量PCR检测Sprouty1mRNA表达，蛋白免疫印迹法检测Sprouty1蛋白表达，酶联免疫法检测IL-2炎症因子的表达。结果①与PCI术前强化组相比，PCI术后强化组Sprouty1mRNA、Sprouty1蛋白表达明显升高（P0.05）③与PCI术前强化组相比，术后强化组IL-2浓度降低（P0.05）。结论强化剂量的阿托伐他汀可以通过上调CD4+T淋巴细胞Sprouty1的表达，从而减少PCI术后心肌的炎症反应。 【关键词】不稳定型心绞痛；阿托伐他汀；CD4+T淋巴细胞；Sprouty1；IL-2 Effects of large dose of atorvastatin on the expression of Sprouty-1 in CD4+T lymphocytes from unstable angina patients during perioperative period of PCI WEN Wei-ming，SU Qiang，WANG Jiang-you，ZHOU You，LIU Yang，LU Yong-guang，LI Lang. Department of Cardiology，The First Affiliated Hospital of Guangxi Medical University，Nanning 530021，China. Corresponding author：LI Lang，Email：drlilang@163.com 【Abstract】ObjectiveTo investigate the effects of large dose of atorvastatin on the expression of Sprouty-1 in CD4+T lymphocytes from unstable angina patients during perioperative period of PCI.MethodsA total of 52 unstable angina patients enrolled were divided randomly（random number） into large-dose atorvastatin （80 mg/d， n=26） pretreated group and moderate-dose atorvastatin （20 mg/d， n=26） pretreated group. Circulating CD4+T cells were obtained by magnetic cell sorting system （MACS） before PCI and 18-24h after PCI. For detecting the gene expression， the reverse transcription fluorescent quantitative polymerase chain reaction （RT-PCR） was used to measure the expression of Sprouty-1 mRNA in CD4+ T lymphocyte. The level of Sprouty-1 protein was detected with Western blot analysis and IL-2 was quantified by enzyme-linked immunosorbent assay （ELISA）. Results①Compared with large-dose group before PCI，the expression of Sprou