环境微生物可培养性影响因素及培养方法研究进展.pdfVIP

21 卷 2 期 生命科学研究 灾燥造援21 晕燥援2 年 月 圆园17 4 蕴蚤枣藻 杂糟蚤藻灶糟藻 砸藻泽藻葬则糟澡 Apr. 圆园17 综 述 DOI ：10.16605/ki.1007-7847.2017.02.0 11 窑 窑 环境微生物可培养性影响因素及培养方法研究进展 * 李晓丹 屈建航 张璐洁 海峰 , , , (河南工业大学生物工程学院，中国河南 郑州450001) 要 目前, 已知并能利用的微生物只是自然界微生物很小的缩影, 过低的可培养效率使人类在认识和利用微 : 生物上处于瓶颈阶段 现综述了微生物活的非可培养状态及其检测方法, 阐述了微生物可培养性的影响因素, 探 讨了提高微生物可培养效率的培养策略和方法进展, 以期为提高微生物的可培养性和菌种资源开发提供依据 关键词 微生物; 可培养性; 活的非可培养(VBNC)状态; 分离培养技术; 菌种资源 : 中图分类号 Q93 文献标识码 A 文章编号 1007 7847(20 17)02 0154 05 : : : - - - Progresses on the Influence Factors of Culturability and Cultivation Strategies of Environmental Microorganisms * LI Xiao-dan, QU Jian-hang , ZHANG Lu-jie, LI Hai-feng (College of Biological Engineering, Henan Unive rs ity of Technology, Zhengz hou 45000 1, Henan, China) Abstract: At present, only a small part of the environmental microorganisms can be recognized and utilized, and the culturable efficiency becomes a bottleneck in making use of the microbial resources. To provide a ba sis for improving the culturability of microorganisms in the environment, the viable but non-culturable (VB NC) state and the detection methods, and influence factors of the culturability of microorganisms were sum marized; the methods for improving the culturable efficiency were discussed. Key words: microorganisms;