妊娠糖尿病患者血清apelin水平及相关因素分析-第三军医大学学报.docVIP

妊娠糖尿病患者血清Apelin水平及相关因素分析( 　 李金超 任伟 李素芳 刘静婧 【摘要】 目的 比较妊娠糖尿病患者与健康妊娠个体血清Apelin水平及影响因素。方法 采用酶联免疫吸附（ELISA）法检测33例妊娠糖尿病患者和30例妊娠期糖耐量正常者的血清Apelin水平，同时检测空腹血糖、血脂谱、空腹胰岛素（FINS）等，计算体重指数（BMI），并以稳态模型评估胰岛素抵抗指数（HOMA-IR）。 结果 妊娠糖尿病组（201±126ng/L）与正常对照组（173±95 ng/L）比较，血清Apelin没有显著差异（P﹥0.05）。多元回归分析显示，Apelin与BMI、空腹血糖、血脂、胰岛素等临床和实验指标之间不存在线性相关。 结论 本研究显示,血清Apelin与GDM之间不存在联系。 【关键词】 妊娠糖尿病；Apelin；葡萄糖耐量 Analysis of serum apelin level and related factors in gestational diabetes mellitus LI jin-chao, REN Wei, LI su-fang, LIU jing-jing.The First Hospital Affiliated to Chongqing University of Medical Sciences, Chongqing 400016，China 【Abstract】Objective In this study, we compared serum Apelin concentrations and the relationship of serum Apelin with effect factors were observed in patients with gestational diabetes(GDM) and women with normal glucose tolerance(NGT). Methods The levels of fasting serum Apelin was measured by enzyme-linked immunosorbent assay(ELISA) in 33 patients with gestational diabetes(GDM) and 30 women with normal glucose tolerance(NGT). Meanwhile, fasting plasma glucose(FPG),lipid profile and fasting insulin level were also measured. BMI were evaluated and insulin sensitivity was accessed by HOMA-IR. Results There were no significant differences in serum apelin levels between the women with GDM and NGT (201±126 ng/L VS 173±95 ng/L，P﹥0.05). Multiple regression analysis with Apelin as a dependent value revealed that serum Apelin levels were no associated with any of the clinical or metabolic parameter analyzed (Age, Gestational age, BMI, Glucose, lipid, Fasting insulin ). Conclusion Circulating Apelin would not relate with GDM in our study. 【Keyword】Gestational diabetes mellitus；Apelin；Glucose tolerance Apelin是G蛋白偶联受体（）的天然内源性配体，APJ 受体及其内源性配体Apelin在体内分布广泛，在中枢与外周组织均有不同水平的表达【1】，并通过作用于APJ参与调解心血管功能、机体免疫、能量代谢等。近年来发现它是一种新的脂肪因子，在鼠类和人类的皮下脂肪细胞均有表达，并受胰岛素的调控【2，3】。在糖尿病动物模型、正常、肥胖及2型糖尿病人群临床研究中均已证实Apelin与肥胖胰岛素抵抗有关。然而，Apelin在妊娠糖尿病人群中的研究甚少，与其他代谢指标的关系亦不明确。本研究通过测定妊娠糖尿病血浆Apelin水平，来了解与血糖、胰岛素抵抗、血脂