外源性生长激素对运动大鼠骨骼肌形态及代谢机能影响.docVIP

外源性生长激素对运动大鼠骨骼肌形态及代谢机能影响摘 要：目的：旨在观察生长激素对运动大鼠骨骼肌形态及机能的影响。方法：28只雄性成熟SD大鼠在适应性训练后随机分成安静对照组、安静注射组、运动对照组和运动注射组。运动组和运动注射组进行跑台训练8周。皮下注射rhGH。结果：1）注射生长激素后，出现了肌纤维排列松散，细胞核数量减少且体积变小，骨骼肌细胞间质水肿等现象。2）在8周的训练后，肌纤维横截面积稍有下降，但变化不明显。注射生长激素4周后，安静组和运动组均显著增加，且安静注射组大鼠的肌纤维横截面积显著高于运动注射组。3）运动组及运动注射组骨骼肌IGF-I明显升高。安静注射组及运动注射组血清IGF-I明显升高，运动组无明显变化。4）各组肌肉总蛋白含量有所增加，分别为安静注射组升高4.77%、运动组升高31.13%、运动注射组升高28.06%。5）运动组及运动注射组腓肠肌ATP酶活性升高。结论：1）8周耐力运动明显促进大鼠骨骼肌蛋白质合成，同时增加了骨骼肌IGF-I水平。2）外源性rhGH注射4周后，大鼠骨骼肌细胞形态产生肥大，骨骼肌肌纤维横截面积明显增加。3）外源性rhGH注射4周可以显著提高血清和骨骼肌IGF-I水平，且骨骼肌总蛋白的含量变化与骨骼肌IGF-I的变化趋势平行。4）外源性rhGH注射4周可以对肌纤维类型及分配产生调节作用，从而改变肌肉代谢酶活性。 关键词：生长激素；胰岛素样生长因子I；运动；骨骼肌 中图分类号：G804.2文献标识码：A文章编号：1007-3612(2011)06-0045-05 Effects of rhGH and Exercise on Morphology and Metabolism Function of Skeletal Muscle in Rats ZHU Han?1, ZHAO Hua?2 (1.Beijing Sport University, Beijing 100084, China;2.Physical Education Department, Tianshui Normal University, Tianshui 741001, Gansu China) Abstract:Objective: The research mainly probes into the effect of rhGH on the skeletal muscle morphology and metabolism function of training rats. Methods: 28 male SD rats were randomly divided into 4 groups: rest group, injection group, exercise group, and exercise plus injection group. The exercise group and exercise plus injection group performed closely monitored training in treadmills for 8 weeks. rhGH was injected subcutaneously. Results: 1) We find that after 4 weeks rhGH injection, skeletal muscular fiber hypertrophy was discovered in injection group. It also finds that there were some morphological changes after morphological observation in most objects, such as cytoplasmic edema. 2) After 8 weeks exercise, the cross-sectional areas of skeletal muscle slightly decrease.After 4 weeks rhGH injection, the cross-sectional area of the skeletal muscle in injection and exercise plus injection group increase significantly. The cross-sectional area of the skeletal muscle in injection was much higher than the exercise plus injection group. 3) T