荔枝核总黄酮对大鼠肝星状细胞增殖的抑制作用及对蛋白TGF-β1和α-SMA表达的影响-内科学专业毕业论文.pdfVIP

用, 其作用机制可能通过抑制细胞TGF-β 和а-SMA 蛋白表达，达到 1 阻滞细胞增殖周期和诱导细胞凋亡，从而达到抗纤维化的作用。 关键词：荔枝核总黄酮；大鼠肝星状细胞；增殖抑制；TGF-β ； 1 а-SMA The effect of Litchi flavonoids on proliferation of rat hepatic stellate cells and the expression of protein TGF-β1 and а-SMA ABSTRACT Objective: The aim of this study was to investigate the molecular mechanism and the effect of growth inhibition on Litchi flavonoids (TFL) to rat hepatic stellate cells (HSC-T6). Methods: MTT assay was used to test Rat hepatic stellate cells (HSC-T6) growth inhibition, and the cell apoptosis was observed after stained with Acridine orange; Cell cycle was detected by Flow cytometry. Results: A variety concentration of Litchi flavonoids (TFL)can inhibit growth activity on rat hepatic stellate cells (HSC-T6) in different time (P0.05),and the half inhibitory concentration was 101.98mg/mL ；In the groups treated by TFL, nuclear enrichment, nuclear breakdown and dense-stained bright green apoptotic bodies were found . Application of flow cytometry and drug treatment control group the distribution of cell cycle ，the test results show that ，Control cells G phase cells as 47.68 ± l 2.92% ，G2 phase cells as 19.58 ±2.93% ，S phase cells as 32.75 ±2.09% ； drug group by Litchi flavonoids (TFL) treatment ，G1 phase cells increased, while the G2 phase cells were significantly reduced, the cells were obviously arrested at G phase （P0.05 ）. Western Blot detection of 2 cells in the application of TGF-β1 and а-SMA protein expression showed that, With the increase of drug concentration, TGF-β1 a