长穗偃麦草1e附加系与杀配子染色体2c附加系杂交-中国农学通报.pdfVIP

中国农学通报 2012,28(15):83-86 Chinese Agricultural Science Bulletin 长穗偃麦草1E附加系与杀配子染色体2C附加系 杂交后代的细胞学及形态学分析 刘春影，杨 蕾，王 丹，郭长虹 （哈尔滨师范大学生命科学与技术学院/黑龙江省分子细胞遗传与遗传育种重点实验室，哈尔滨150025） 摘 要：长穗偃麦草是小麦的野生近缘种属，具有抗寒、抗旱、抗病等优异性状。为了利用长穗偃麦草的 优异基因，将‘中国春’-长穗偃麦草1E 二体附加系与‘中国春’-柱穗山羊草2C 二体附加系杂交、自交和 回交，观察其后代的细胞学和形态学特性。结果表明，在杂交后代有丝分裂和减数分裂中观察到了染色 体畸变现象；统计F 代自交结实率，发现与亲本相比，这些杂种后代的结实率明显降低；杂种后代的穗型 1 发生了分离，除正常穗型外还观察到了密穗型，说明杀配子染色体2C 可以诱导染色体畸变并对结实率 和穗型均有一定的影响。本研究为进一步创制小麦-长穗偃麦草1E 染色体易位系和缺失系奠定了基 础。 关键词：小麦；长穗偃麦草；细胞学；形态学 中图分类号：Q37 文献标志码：A 论文编号：2012-0012 CytologicalandMorphologicalCharacteristicsofHybridsBetweenLophopyrumelongatum1E AdditionLineandGametocidalChromosome2CAdditionLine Liu Chunying, Yang Lei, Wang Dan, Guo Changhong (KeyLaboratoryofMolecularCytogeneticsandGeneticBreedingofHeilongjiangProvince/ CollegeofLifeScienceandTechnology,HarbinNormalUniversity, Harbin 150025) Abstract: Lophopyrum elongatum is a wild relative of wheat that has excellent traits such as cold resistance, drought resistance and disease resistance. In order to transfer the desirable genes of Th. elongatum into wheat, hybrid, selfing and backcross were done between ‘Chinese spring ’-Th. elongatum 1E disomic addition line and ‘Chinese spring ’-Ae. cylindrica gametocidal chromosome 2C disomic addition line, the cytological and morphological characteristics of offspring were observed. Chromosome aberrations were observed in the mitosis and meiosis, the seed-setting rate of F was lower significantly compared with parents, the spike separation was 1 observed in the hybrids, in addition to the normal spike, we also observed the compactoid.