低温胁迫对小麦叶片细胞膜脂质过氧化产物及相关酶-麦类作物学报.pdfVIP

麦类作物学报 　2006 ,26 (4) :70～73 J ournal of Triticeae Crop s 低温胁迫对小麦叶片细胞膜脂质过氧化产物 及相关酶活性的影响 刘艳阳 ,李俊周 ,陈　磊 ,崔党群 (河南农业大学农学院 ,河南郑州 450002) 摘 　要 : 为了给小麦抗寒性鉴定提供依据 ,采用室内模拟低温处理小麦 133 个系的 D H 群体幼苗 ,研究 了低温胁迫对小麦幼苗叶片脂质过氧化作用的产物及其相关酶活性的影响 。结果表明 ,低温处理后脂质过氧 ( ) ( ) ( ) 化作用产物丙二醛 MDA 的含量增加 ;超氧物歧化酶 SOD 和过氧化物酶 POD 的活性在低温胁迫条件下 不同株系间存在差异 ,但并不随 MDA 的增加而稳定提高 ,两种酶之间的活性变化无明显的同步性 。因此 , SOD 活性和 MDA 含量可以作为鉴定小麦抗寒性的生理生化指标 。 　　关键词 : 小麦 ;低温胁迫 ;膜脂质过氧化 ; SOD 酶活性 ; POD 酶活性 　　中图分类号 :S5 12 . 1 ; S3 11 　　　　文献标识码 : A 　　　　　文章编号 (2006) Eff ect of Low Temperature Stress on Peroxidation Product of Membrane L ipids and Activity of Related Enzymes in Wheat Seedl ing Leaves L IU Yanyang ,L I Junzhou , CHEN Lei , CUI Dangqun (Agronomy College , Henan Agricult ural U niver sit y ,Zhengzhou , Henan 450002 , China) Abstract : The p ero xidation p ro duct of membrane lipids an d activit y of relat ed enzyme s in w heat do ub led hap loid pop ulation seedling leave s under low t emp erat ure st re ss were st u died . The relatio n ship be t ween col d re si st ance of w heat and membrane sy st em were st u died synchronou sly . The re sult s are t hat t he low t emp erat ure st re ss increa sed t he p ero xidation p ro duct of membrane lip ids MDA ,t he relat ed def en sive enzyme s SOD and POD did not increa se st ably wit h MDA . The chan ge s of t he activitie s of SOD and POD hadn’t showed t he obviou s synchronization . They har monized wit h each ot her under low t emp erat ure st re ss. The activitie s of SOD and cont ent of MDA can be u sed a s indexe s to det ect t he cold re si st ance