食品中牛奶过敏原成分lamp检测方法的建立 establishment of loop-mediated isothermal amplification method for detection of milk allergen in foods.pdfVIP
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食品中牛奶过敏原成分lamp检测方法的建立 establishment of loop-mediated isothermal amplification method for detection of milk allergen in foods
5 4 Vol. 5 No. 4
2014 4 Journal of Food Safety and Quality Apr. , 2014
LAMP
1,2 1 1 1 2*
程晋霞 , 曾 静 , 马 丹 , 魏海燕 , 张海予
(1. , 100026; 2. , 102206)
摘 要: 目的 LAMP(loop-mediated isothermal amplification),
PCR(real-time PCR)方法 b(cyt-b)LAMP
, real-time PCR 结果 LAMP 9
, , 0.5%,
real-time PCR 69 , real-time PCR 结论
LAMP , , ,
,
关键词: ; ; (LAMP); PCR
Establishment of loop-mediated isothermal amplification method for
detection of milk allergen in foods
1,2 1 1 1 2*
CHENG Jin-Xia , ZENG Jing , MA Dan , WEI Hai-Yan , ZHANG Hai-Yu
(1. Beijing Entry-Exit Inspection and Quarantine Bureau, Beijing 100026, China;
2. Beijing University of Agriculture , Beijing 102206, China)
ABSTRACT: Objective The loop-mediated isothermal amplification (LAMP) detection method was
established for milk allergen testing and compared with the real-time PCR detection methods. Methods
The cyt-b gene of bovine was used to design LAMP primers and then establish reaction system. The spe-
cificity and sensitivity of LAMP were compared with real-ti
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