尾巨桉家系外植体诱导技术-国家林业局桉树研究开发中心.pdfVIP

28 1 Vol. 28 No. 1 2011 6 EUCALYPT SCIENCE TECHNOLOGY Jun . 20 11 邓海群, 莫继有 , 玉首杰, 石 前 ( , 532108 : , : 5 , , 38% , , 62% ; 1 , , , 68% ; , 32% ; 1. 2 mg L - 1 BA, 0. 6 mg L - 1 NAA , , 2~ 3 , ; , , , , : ; ; ; : S722. 3+ 7 : A InductionTechni ues of Eucal tus uro h lla E. randis Families Ex lant q yp p y g p DENG Hai- qun, MO Ji- you, YU Shou- jie, SHI Qian ( Guangxi Dong men Fores t Farm, Fusui 532108, Guangx i, China Abstract: Related techni ues includin retreatments methods, disinfectin rea ents, materials, culture medi q g p g g um and seasons for inducing explants of E. urop hylla E. grandis families were carried out. The results showed that the best retreatment method was to soak ex lants b saturated deter ent for 5 minutes and then p p y g to clear the surface pollutants by brush, which could get the lowest polluted rate of 38% and the highest germi natin rate of 62% ; the best disinfectin rea ent was alcohol with H Cl2 used to disinfect one time, which g g g g could have lower olluted and burned rate and the hi hest ermination rate of 68% ; the best materials for induc p g g ing was sprouts after cutting trees, which had good germinating rate of 32% ; the best hormone concentration of culture medium was 1. 2 m L - 1 BA with 0. 6 m L- 1 NAA which could et a ro riate ro a atio