蕙兰floweringlocust基因的克隆及其对开花的影响-天恩泽.pdfVIP

2013,46(7):1419-1425 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2013.07.012 310021 Isolation of Flowering Locus T Ortholog and the Effects on Blooming of Cymbidium faberi SUN Chong-bo, XIANG Lin, LI Xiao-bai, QIN De-hui, LI Bo-jun, GUO Fang-qi, WU Chao (Institute of Horticulture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021) Abstract: ObjectiveThe objective of this study was to analyze the function of Flowering locus T(FT) on blooming in Cymbidium faberi. Method The full-length cDNA sequence of CfFT was obtained by RT-PCR and RACE cloning technology. The expression patterns of CfFT in different tissues and flower buds of different developmental stages were analyzed by real-time quantitative RT-PCR. To investigate the function of CfFT, an expression vector was constructed for transformation into tobacco by using agrobacterium-mediated method.ResultIn flowers, CfFT was expressed more in young flower buds than in mature flowers and was predominantly expressed in young ovary. Ectopic expression of CfFT in transgenic tobacco plants showed novel phenotypes by flowering earlier than wild-type plants. Real-time quantitative RT-PCR analysis suggested that expression levels of CfFT closely related with the flowering time of different 35S::CfFT transgenic tobacco lines. Further analysis of the flower time related genes indicated that the expression of genes LEAFY (LFY), APETALLA1 (AP1), FRUITFULL (FUL) and SEPALLATA1 (SEP1) were closely related with the expression of FT in 35S::CfFT transgenic tobacco plants.ConclusionEctopic expression of CfFT gene promote flowering in transgenic tobacco. Key words: Cymbidium faberi; FT gene; flower induction; tobacco; real time quantitative RT-PCR