不同分散温度时间对双波长法测定高粱直支链淀粉-中国农学通报.PDF

中国农学通报 2014,30(18):245-250 Chinese Agricultural Science Bulletin 不同分散温度、时间对双波长法测定高粱直、 支链淀粉及总淀粉含量的影响 刘选选，曹国璠，任明见，张 君，李祥栋，陈 杏 （贵州大学农学院，贵阳550025） 摘 要：为了测定高粱直、支链淀粉及总淀粉含量，采用双波长比色法研究了不同分散温度、时间处理对 高粱淀粉直、支链淀粉及总淀粉含量的影响。结果表明，在试验温度70~ 100℃范围内，75℃分散15 min 时支链淀粉百分含量最高，85℃分散5 min 时支链淀粉百分含量最低。对于直链淀粉，95℃以后，随着分 散的时间延长，测得的高粱直链淀粉百分比不断增高。样品分散温度与时间对高粱中总淀粉含量影响 与支链淀粉相同，其测定值在75℃分散15 min 时最高。由此可知，不同分散温度、时间处理对双波长法 测定高粱淀粉直、支链淀粉及总淀粉含量影响不一样。 关键词：高粱；双波长；支链淀粉；直链淀粉；分散温度；分散时间 中图分类号：S1;S512.1 文献标志码：A 论文编号：2013-2589 TheEffectofDifferentSampleDispersionTemperaturesandTimeonSorghumAmyloseand Amylopectin,TotalStarchContentDeterminationbyDoubleWavelengthSpectrophotometricMethod LiuXuanxuan,CaoGuofan,RenMingjian,ZhangJun,LiXiangdong,ChenXing (CollegeofAgriculture,GuizhouUniversity,Guiyang550025) Abstract: Inordertodeterminetheamyloseandamylopectin,totalstarchcontentofsorghum,theeffectof differentsampledispersiontemperaturesandtimesonSorghumamyloseandamylopectin,totalstarchcontent by double wavelength spectrophotometric method were studied by double wavelength spectrophotometric method.Intherangeof70-100℃,thepercentageofamylopectinwasthehighestwhenthesampledispersion temperatureandtimewere75℃and 15min,thepercentageofamylopectinwasthelowest,amongthesample dispersiontemperatureandtimewere 85℃and5min.Fortheamylose,thelongerofdispersion,thehigherof percentage,after90℃.Whenthesampledispersiontemperaturewas75℃,thedeterminedtotalstarchcontent wasthehighest.Theseresultsdemonstratedthattheeffectsofsampledispersiontemperaturesandtimesonthe determinationofamylose,amylopectinandtotalstarchcontentweredifferent. Keywords: sorghum;doublewavelengths;amylase;amylopectin;temperatureofthedispersion;timeofthe dispersion 0 引言