包含金纳米粒子的聚电解质微囊的制备Preparationof-药学学报.PDFVIP

药学学报 Acta Pharmaceutica Sinica 2010, 45 (3) : 371375 ·371 · 包含金纳米粒子的聚电解质微囊的制备 * 孙雅洁, 朱家壁, 郑春丽 ( 中国 科大学 剂研究所, 江苏 南京 210009) 摘要: 本文制备了含金纳米粒子的聚电解质微囊, 并进行了表征。以碳酸钙粒子为模板, 在其表面组装聚烯 丙基胺盐酸盐 [poly (allyamine hydrochloride), PAH] 和金纳米粒子, 得到以碳酸钙粒子为母核, PAH/金纳米粒子 为壳的核壳结构微粒。用乙二胺四乙酸二钠 (EDTA) 溶解碳酸钙, 即可得到含有金纳米粒子的聚电解质微囊。 用扫描电镜表征碳酸钙粒子、含金纳米粒子的聚电解质微囊去掉母核前后的形状, 可观察到碳酸钙粒子表面包 裹金纳米粒子前后的差别。用显微镜表征了微囊在溶液中的形态, 微囊在水中分散 良好。载入异硫氰酸荧光 素标记的牛血清白蛋白 (FITC-bovine serum albumin, FITC-BSA) 作为模型 物, 用荧光显微镜可以观察到微囊 内有一定的荧光强度, 检测得到牛血清白蛋白的包封率为 (34.31 2.44) %, 聚电解质微囊载 量为 (43.75 3.12) mg·g−1。 关键词: 金纳米粒子; 聚电解质微囊; 迭层自组装技术 中图分类号: R943 文献标识码:A 文章编号: 05 13-4870 (2010) 03-0371-05 Preparation of polyelectrolyte microcapsules contained gold nanoparticles * SUN Ya-ji e, ZHU Jia-bi , ZHENG Chun-li (Pharmaceutical Research Institute, China Pharmaceutical University, Nanj ing 210009, China) Abstract: In this work, polyelectrolyte microcapsules containing gold nanoparticles were prepared via layer by layer assembly. Gold nanoparticles and poly (allyamine hydrochloride) (PAH) were coated on the CaCO3 microparticles. And then EDTA was used to remove the CaCO3 core. Scanning electron microscopy (SEM) was used to characterize the surface of microcapsules. SEM images indicate that the microcapsules and the polyelectrolyte multilayer were deposited on the surface of CaCO3 microparticles. FITC-bovine serum albumin (FITC-BSA, 2 mg) was incorporated