大鼠自体完整脾脏粘贴移植于肝脏表面后的再生研究马海军韩冰樊超.DOC

大鼠自体完整脾脏粘贴移植于肝脏表面后的再生研究 马海军 韩冰 樊超 刘仕琪 万真 师建华 王善佩 吕毅 西安交通大学第一附属医院肝胆外科，陕西 西安 710061 摘要 目的 观察大鼠自体完整脾脏粘贴移植于肝脏表面后的再生效果。方法 将120只SD大鼠随机分成12组，完整切除脾脏并称重，然后用医用胶将脾脏贴于肝左外叶膈面1、3、、、、、、、、6只分离肝脏表面移植的脾脏称脾脏术后基本恢复44.2%±3.6%）。移植于肠系膜的脾脏组织在术后56 d可观察到典型的红髓和白髓结构，术后84 d边缘区的结构仍不清晰。结论 肝脏可作为脾脏自体移植的部位，移植于肝表面的脾脏的组织学再生优于肠系膜；医用胶粘贴法有望成为脾组织自体移植的新方法。 关键词 脾脏；移植；肝脏；再生；医用胶 中图法分类号：R332 Study of the regeneration of spleen auto-transplanted by tissue adhesive on the liver surface in rats MA Hajun，HAN Bing，FAN Chao，LIU Shiqi，Wang Zhen， SHI Jianhua，WANG Shanpei， LV Yi. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Medical College, Xi’an Jiaotong University, Shaanxi, 710061, China. Corresponding author: LV Yi，E-mail：luyi169@126.com Abstract Objective To investigate the effects of the regeneration of spleen auto-transplanted by medical adhesive on the liver surface in rats. Methods 120 Sprague-Dawley rats were randomly divided into twelve groups. After splenectomy, each spleen was weighed and attached to the diaphragmatic side of the left hepatic lobe using an adhesive. The rats were accordingly sacrificed on postoperative day(POD)1, 3, 7, 14, 21, 28, 35, 42, 49, 56, 70 and 84. Six spleens in each group were dissected for weighing and the other four for histological analysis(HE). The splenic autografts of another 12 rats were attached to mesenterium. The rats were accordingly sacrificed on POD28, 56 and 84 for histological analysis(HE). Results The spleens attached on liver underwent almost complete necrosis and then regenerated centripetally into a structure comparable to normal spleen, with typical red and white pulps as well as marginal zone on POD35. The mass of the implants recovered to more than two fifths of the original mass(44.2%±3.6%) on POD84. For the splenic autografts attached to mesenterium, the typical red and white pulps were observed on POD56. The marginal zone was not clear on POD84. Conclusion Liver is proved to be an appropriate site for spl