小麦阿拉伯木聚糖对S180荷瘤小鼠免疫活性及肠道代谢产物地影响.pdfVIP

现代食品科技 Modern Food Science and Technology 2015, Vol.31, No.10 小麦阿拉伯木聚糖对S180荷瘤小鼠免疫活性及 肠道代谢产物的影响 赵梦丽，王丽丽，刘丽娅，钟葵，佟立涛，刘兴训，周闲容，周素梅 （农业部农产品加工综合性重点实验室，中国农业科学院农产品加工研究所，北京 100193） 摘要：本文研究了碱提酸水解阿拉伯木聚糖(AX1)和水提酸水解阿拉伯木聚糖(AX2)对 S180 荷瘤小鼠机体免疫水平及肠道代谢 产物的影响。成分分析表明 AX1 的蛋白质(1.50%)与阿魏酸含量(7.6 μg/g AX1)均低于AX2(蛋白质 7.97%，阿魏酸366.3 μg/g AX2)。 二者的重均分子量(Mw)较为接近，约 3×104 u。与阴性对照组相比，AX 样品可以抑制小鼠肿瘤的增长，特别是 AX2 ，其抗肿瘤能力 与灵芝多糖组(GLP)组相当；AX1 、AX2 均可显著提高小鼠NK 细胞的活性，且AX2 的作用效果比AX1 好；经 AX1 与AX2 灌胃给 药的小鼠腹腔巨噬细胞吞噬作用均有所增强，其中 AX2 组作用效果与 GLP 相当；此外，与阴性对照组相比，GLP、AX1 、AX2 对 DTH 反应具有明显效果(P0.05)。采用气相色谱法(GC)分析小鼠结肠内容物中短链脂肪酸含量，结果显示 AX1 和AX2 组的短链脂肪 酸显著高于阴性对照组和灵芝多糖组(P0.05)。 关键词：阿拉伯木聚糖；抗肿瘤；免疫活性；短链脂肪酸 文章篇号：1673-9078(2015)10-1-6 DOI: 10.13982/j.mfst.1673-9078.2015.10.001 Effect of Wheat Arabinoxylan on the Immunological Activity and Intestinal Metabolites of S180 Tumor-bearing Mice ZHAO Meng-li, WANG Li-li, LIU Li-ya, ZHONG Kui, TONG Li-tao, LIU Xing-xun, ZHOU Xian-rong, ZHOU Su-mei (Key Laboratory of Agro-products Processing, Ministry of Agriculture, Institute of Agro-products Processing Science Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China) Abstract: Acid-hydrolyzed arabinoxylans extracted with alkali (AX1) and water (AX2) were prepared to study their effects on the immune activity and intestinal metabolites in S180 tumor-bearing mice. Chemical analysis showed that both the protein content (1.50%) and ferulic acid content (7.6 μg/g) of AX1 were lower than those of AX2 (protein, 7.97%; ferulic acid, 366.3 μg/g). The weight-average molecular weights (Mw) of AX1 and AX2 were similar and were about 3 × 104 u. Compared with the negative control group, AX samples could inhibit the tumor growth