乌金胶囊中延胡索乙素含量的测定-第二军医大学学报.docVIP

高效液相色谱法测定肝力保胶囊中苦参碱及槐定碱的含量 王 慧，路克军，张国庆﹡ 第二军医大学东方肝胆外科医院药材科 上海 200438 [摘要] 目的 建立高效液相色谱法测定肝力保胶囊中苦参碱及槐定碱含量。方法 使用Agilent NH2 (4.6 mm×250 mm, 5μm)，流动相：1%磷酸:无水乙醇:乙腈=10:22:68，流速0.8 ml/min，柱温25℃，检测波长210nm。结果 苦参碱在5.21-156.3 μg/ml （r=1），槐定碱在5.29-158.7 μg/ml（r=0.9999）浓度范围内呈良好线性关系，苦参碱回收率为98.73% (RSD=3.67 %)，槐定碱回收率为100.10%（RSD=2.24%）。15批肝力保胶囊中苦参碱含量在9.04-13.4 1 mg/粒之间，槐定碱含量在6.48-15.62 mg/粒之间。结论 该方法简便、准确，重复性好，可用于肝力保胶囊的质量控制。 [关键词] 高效液相色谱法；苦参碱；槐定碱；肝力保胶囊；含量测定 Determination of matrine and sophoridine contents in Ganlibao capsule by High Performance Liquid Chromatography WANG Hui, LU Ke-jun，ZHANG Guo-qing﹡ (Department of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438) [Abstract] Objective To determination of matrine and sophoridine contents in Ganlibao capsule by HPLC method. Methods Agilent NH2 (4.6 mm?50 mm, 5fμm) column was used with 1% phosphoric acid: ethanol: acetonitrile (v/v=10:22:68) as the mobile phase. The flow rate was 0.8 ml/min, and the temperature was at 25℃.The detection wavelength was 210 nm. Results The matrine calibration curve was at the range of 5.21-156.3 μg/ml(r =1), and the sophoridine calibration curve was at the range of 5.29-158.7 μg/ml(r =0.9999). The recovery of matrine was 98.73% (RSD=3.67 %) ,and the recovery of sophoridine was 100.10% (RSD=2.24 %).15 batches of Ganlibao capsule matrine contents were 9.04-13.41mg/tablet, and sophoridine contents in Ganlibao capsule were 6.48-15.62 mg/tablet. Conclusion The method is simple, accurate and reproducible, which can be used for the quality control of Ganlibao capsule. [Key words] HPLC; matrine ;sophoridine; ganlibao capsule; determination 肝力保胶囊是我院自制制剂，由茵陈、虎杖、茯苓、黄芪及苦参碱提取物组成。临床用于各种急、慢性病毒性肝炎治疗，对肝癌所致肝脾肿大、肝硬化腹水等症状有辅助治疗。苦参为豆科植物苦参Sophora flavescens Ait.的干燥根，具有清热燥湿、杀虫、利尿的功效。苦参中含有生物碱、黄酮类化合物、三萜类成分、蒽醌类成分等，其主要功效成分生物碱在中枢神经系统、心血管系统、抗病毒、抗炎、抗肿瘤、免疫调节等方面都有重要的药理活性的临床功用[1-3]。肝力保胶囊在我院使用多年，临床效果好，目前其质量控制标准包括黄芪甲苷定性及苦参碱含量限定，本文以苦参提取物为研究对象，建立HPLC法同时测定肝力保胶囊中苦参碱和槐定碱含量，该方法专属性强、准确度高、