发育温度对蜜蜂归巢能力及记忆相关基因表达的影响-应用昆虫学报.pdfVIP

Chinese Journal of Applied Entomology 2016, 53(6): 12611266. DOI: 10.7679/j.issn.20951353.2016.157 发育温度对蜜蜂归巢能力及记忆 相关基因表达的影响* ** *** 袁 安 郭亚惠 黄 晓 廖春华 吴小波 330045 摘 要 【目的】 Apis mellifera ligustica 【方法】3 48 h3 33℃35℃ 37℃75%±5%1 1 000 m2 000 m 10 20 3 [A Glu-RA N- -D- 1 Nmdar1 1 tyr1]【】 1 000 m 2 000 m 10 20 GluRA Nmdar1 Tyr1 10 20 Tyr1 【结论】 关键词 Effect of brood developmental temperature on the homing capability and memory related gene expression of worker bees Apis mellifera L ． ** *** YUAN An GUO Ya-Hui HUANG Xiao LIAO Chun-Hua WU Xiao-Bo (Honeybee Research Institute, Jiangxi Agricultural University, Nanchang 330045, China) Abstract [Objectives] The objective of this experiment was to explore the effects of developmental temperature on the homing capability and memory related gene expression levels of worker bees Apis mellifera L. [Methods] Three honeybee colonies of equal population size were used in experiments. Each queen was confined within an empty frame to lay eggs for 48 hours. Each frame was then separated into three equal sections after larval cells had been capped and the three sections were placed in different incubators and incubated at either 33, 35, or 37 ℃, until hatching took place. Relative humidity in each incubator was 75%±5%. New born worker bees from each incubator were marked with different colors and returned into their natal colonies. The homing rates