盐酸小檗碱对体外培养人牙周膜细胞炎性反应影响的初步分析刘鑫1.docVIP

盐酸小檗碱对体外培养人牙周膜细胞炎性反应影响的初步分析 刘 鑫1，贾 艳2，张 莉2，董天贞2，邓蔓菁2* （1、重庆市第三人民医院科教处 重庆 400014；2、第三军医大学附属第三医院野战外科研究所口腔科 重庆 400042） 【摘要】目的：研究盐酸小檗碱（Ber）作用于体外培养的人牙周膜细胞（PDLCs），在脂多糖（LPS）刺激下，合成和分泌前列腺素E2（PGE2）、白细胞介素-IL-1β）和白细胞介素-6IL-6）的变化，初步推断Ber对牙周炎的治疗作用。方法：将体外培养的PDLCs根据加入LPS与否设定为NLPS和LPS两大组，再根据培养液中Ber终浓度0μg/mL、5μg/mL、10μg/mL和20μg/mL将其分为NLPS0、NLPS1、NLPS2、NLPS3以及LPS0、LPS1、LPS2和LPS3共八个组，用ELISA法检测上清液中PGE2IL-6的浓度。PGE2、IL-6和IL-1β有一定的表达量（P0.05）加入LPS后上清液中PGE2IL-1β和IL-6表达明显升高，差异具有统计学意义（P0.05）在加入LPS的同时加入Ber后与组比较PGE2IL-1β和IL-6表达明显下降（P0.05）PGE2表达，LPS各组差异具有统计学意义（P0.05）差异具有统计学意义（P0.05）IL-1β表达（P0.05）（P0.05）（P0.05）IL-6表达，随着Ber浓度升高，各LPS组表达逐渐降低（P0.05）（P0.05）Ber可以抑制LPS刺激PDLCs产生的炎反应E2；白细胞介素-白细胞介素-6reliminary Analysis on the Inflammatory Response of Cultured Human Periodontal Ligament Cells in Vitro under the Effect of Berberine hydrochloride LIU Xin1,JIA Yan2,ZHANG Li2,DONG Tianzhen2,DENG Manjing2* (1.Department of Science and Education, the Third People’s Hospital of Chongqing,Chongqing, 400014;2. Department of Stomatology, Institute of Surgery Research, Daping Hospital, Third Military Medical University, Chongqing 400042) [基金项目]重庆市自然科学基金(2011BA5013) [作者简介]刘鑫，男，硕士，主治医师，主要从事口腔内科学临床及基础研究。E-mail:ares_young@163.com [通讯作者]邓蔓菁，E-mail:iradeng@163.com [Abstract] Objective To evaluate the effect of Berberine hydrochloride (Ber) on periodontitis by detecting the changes in concentration of prostaglandin E2 (PGE2）, interleukin-1β (IL-1β) and interleukin-6 (IL-6)，which were expressed by human periodontal ligament cells (PDLCs) in vitro after simulated by lipopolysaccharide (LPS). MethodsPDLCs cultured in vitro were Divided into NLPS groups and LPS groups, then each group were further divided into four subgroups (NLPS0, NLPS1, NLPS2 and NLPS3; LPS0, LPS1, LPS2 and LPS3), according to their different concentration (0μg/mL, 5μg/mL,10μg/mL and 20μg/mL) of Ber added. And then detected the concentrations of PGE2, IL-1β and IL-6 in the subgroups by ELISA. Results PGE2, IL-1β and IL-6 were detected in every subg