芪仙清鸣颗粒对哮喘小鼠气道炎症的作用及机制赖一鸣倪振华李钊.docVIP

芪仙清鸣颗粒对哮喘小鼠气道炎症的作用及机制 赖一鸣 倪振华 李钊 王雄彪 【摘要】 目的 通过小鼠支气管哮喘模型，探讨芪仙清鸣颗粒治疗支气管哮喘的作用和机制。方法 选取30只健康雌性BALB/c小鼠随机分为正常对照组、哮喘组和芪仙清鸣颗粒治疗组。以卵白蛋白致敏法建立支气管哮喘小鼠模型，造模成功后予药物处理2周，苏木精-伊红(H-E)染色观察病理组织变化，过碘酸一雪夫（PAS）染色检测气道粘液分泌情况，ELISA检测血清IL-4浓度，免疫组织化学法检测PTEN蛋白表达情况。结果 与哮喘组相比，治疗组气道炎性细胞浸润和气道粘液高分泌显著改善、血清IL-4含量显著降低（P=0.035），并且小鼠肺组织PTEN的表达恢复接近正常，与哮喘组相差显著（P=0.013）。结论　芪仙清鸣颗粒能显著抑制哮喘小鼠气道炎症，改善病理损伤。选择性上调人第10号染色体缺失的磷酸酶及张力蛋白同源的基因Effect of QiXianQingMing granule on airway inflammation in a mouse model of asthma Lai Yiming，Ni Zhenghua，Li Zhao，Wang Xiongbiao. Department of Respiratory Diseases,Putuo Hospital,Shanghai University of Chinese Medicine，Shanghai 200062，China　 Corresponding author：WangXiongbiao. E-mail： 【Abstract】 Objective To explore the effect and mechanism of?QiXianQingMing granule in the treatment of bronchial asthma by OVA-challenged mice model. Methods 30 healthy female BALB/c mice were randomly divided into normal control group, asthma group and?QiXianQingMing granule group. Asthma mouse were challenged with OVA and then treated with saline. QiXianQingMing granule group treated with drug after OVA chanllenge. Hematoxylin-eosin staining(H-E) staining was applied to observe the?pathological change,?periodic Acid?Schiff?(PAS)?staining to detect?a?airway mucus?secretion.?ELISA?detection was used to detect the concentration of serum IL-4. Immunohistochemistry?was used to detect the expression of PTEN protein． Results Compared with the asthma group, the airway inflammation and airway mucus hypersecretion is improved in QiXianQingMing granule group ,while the concentration of serum IL-4 became lower（P=0.035）and the expression of PTEN in lung tissue was significantly higher than the asthma group (P=0.013). Conclusion QiXianQingMing granule could significantly improve asthma pathogogy and selectively upregulated PTEN expression. PTEN pathway may be one of the mechanism of QiXianQingMing granule in asthma treatment. 【Key words】Asthma； QiXianQingMing granule； Phosphatase and tensin homolog; a