沙门氏菌环丙沙星诱导株marr, soxr和acrr基因突变与多重耐药性的相关性研究 association of marr, soxr and acrr mutations with multiple antibiotic resistance in ciprofloxacin-selected salmonella mutants.pdfVIP
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沙门氏菌环丙沙星诱导株marr, soxr和acrr基因突变与多重耐药性的相关性研究 association of marr, soxr and acrr mutations with multiple antibiotic resistance in ciprofloxacin-selected salmonella mutants
第1卷第1期 食品安全质量检测技术 VoLlNo.1
2009年lO月 Food and Detection 0ct.2009
SafetyQuality Technology
Associationof andacrRmutationswith antibiotic
marR,soxR multiple
Salmonella
in mutants’
resistance
ciprofloxacin-selected
Li Yongnin94,WuCongmin92,ShenJianzhon92·+
Linl一,WangYupin93,Wu
Science U赫izy,饿向230036,P兄饧跏口;
(JCo/degeofAnimaland%咖越啊,加梳Agricultural
2 Veterinary
D印artmerttof砌∞舳蝴and强嘞幽:gy,Collegeof
College,蚴054000,PIR优m;
3靴Medical
4妣弦Nutrition蒯Food
mutationsin and
Abstract:PointmarO,marR,marA,marB,soxR,soxSacrR(includingpromoterregion)
Salmonellaisolatesfromanimals.Newin
werestudiedin1 1讯vitroselected mutations
ciprofloxacin—resistant
andacrRwere nomutationsin andsoxSwerefoundinthemu—
marR,soxR detected,and marO,marA,marB
tants.ThemRNA levelsof andwICdeterminedreal—timefluorescent
expression marA,soxS,acrA,acrB by
in acrR
the and mutations
upregulatedmutants.marR,soxR
quantitativeRT·PCR(qRT—PCR)weresignificantly
the of andacrAB—tolCand antibiotic
correlatedwith overexpressionmarA,soxS multiple
alsoresistantt
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