棉花无腺体近等基因系差异表达基因分析.pdfVIP

棉 花学 报 Cotton Science 20 15袁27 6 冤院506―514 花无腺体近等基因系差异表达基因分析 鹏飞 陆才瑞 邹长松 程海亮 杨文翠 冯晓旭 张友平 王巧连 宋国立* 国农业科学院棉花研究所/ 棉花生物学国家重点实验室袁河南安阳455000冤 摘要院为了揭示棉花腺体发育的分子机制袁本研究利用RNA-Seq 对棉花无腺体性状近等基因系Z 12/Z 12YW 的幼嫩叶片进行转录组测序袁 寻找差异表达基因遥 转录组测序结果表明袁 与有腺体棉Z 12 相比袁 无腺体棉 Z12YW 中共有306 个基因表达发生变化袁其 282 个基因下调袁24 个基因上调遥 差异表达基因GO Gene on- tology冤功能注释显示袁细胞组分尧胞外基质组分的功能变化以及细胞杀伤生物学过程 腺体形成过程中发挥 重要作用曰COG Cluster of orthologous groups 冤功能和KEGG Kyoto encyclopedia of genes and genomes冤通路显 著性富集发现袁棉酚等萜烯物质的合成主要受甲羟戊酸途径上游基因的表达调控遥 此外袁本研究共筛选到 13 个差异表达转录因子袁可能 腺体发育和棉酚合成过程中发挥关键作用遥 关键词院棉花 L. 冤曰腺体曰近等基因系曰转录组 中图分类号院S562.035 文献标志码院A 文章编号院1002-7807 20 15冤06-0506-09 10.11963/issn.1002-7807.20 1506002 Jiang Pengfei, Lu Cairui, Zou Changsong, Cheng Hailiang, Yang Wencui, Feng Xiaoxu, Zhang Youping, Wang Qiaolian, Song Guoli* ( 455000, ) The expression changes of transcriptome of tender leaves of Z 12/Z 12YW were analyzed by RNA-Seq to reveal the molecular mechanism of gland formation. Z 12/Z 12YW are cotton near-isogenic lines with a common genetic background but contrasting a gland phenotype. A total of 306 differentially expressed genes were uncovered, of which 282 were down-regulated and 24 were up-regulated in the glandless material Z 12YW. Gene Ontology functional annotation showed that the cell part, the extracellular matrix part and the cell killing played important roles in the process of gland development. Clustering of Ortholo- gous groups and Kyoto Encyclopedia of Genes and Genomes enrichment analyses revealed that the upstream key genes of the mevalonate pathway had a great influence on the biosynthesis of gossypol and other terpene compounds. In addition, we screened 13 tran