生物膜处理高含油废水及膜表面微生物群落特性研究-论文.pdfVIP

中国环境科学 2016,36(9) ：2800~2806 China Environmental Science 生物膜处理高含油废水及膜表面微生物群落特性研究 * 林莹莹,聂麦茜 ,王 琰,贺美丽,李 虹 (西安建筑科技大学环境与市政工程学院,陕西 西安 710055) 摘要：利用单一铜绿假单胞菌 NY3 生物膜在敞开体系中(非无菌条件下)处理石油废水,结果发现,生物膜可高效率去除高浓度含油废水中 的油类物质.在 2g/L 含油水样中,连续运行 25d,石油烃去除率可持续保持约 91.1%.停止进水后,曝气可使生物膜活性恢复. 自然环境中,配伍 于 NY3 菌生物膜中的微生物主要有芽孢杆菌、假单胞菌和红球菌属,其中在实验室能够培养出来的微生物经 16SrRNA 鉴定分别属于红球 菌属和芽孢杆菌属,经检测,2 株菌均有高的石油烃降解效率. 关键词：铜绿假单胞菌 NY3 ；固定化生物膜；高浓度含油废水；微生物群落 中图分类号：X172,X703 文献标识码：A 文章编号：1000-6923(2016)09-2800-07 Study on the high concentration oil-containing wastewater by biofilm treatment of P.aeruginosa strain NY3 * and the characteristics of microbial community in biofilm. LIN Ying-ying, NIE Mai-qian , WANG Yan, HE Mei-li, LI Hong (School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an 710055, China). China Environmental Science, 2016,36(9) ：2800~2807 Abstract ：Single P. aeruginosa NY3 can grow and be immobilized on the surface of polyurethane foam carrier, the biofilm was used in an open system (non sterile conditions) to treat the oil-containing wastewater. The results showed that the biofilm can efficiently removeoil material of high concentrationoily wastewater. After continuously running for 25 d in the oil-bearing wastewater of 2g/L, the removal rate of petroleum hydrocarbons can be maintained at 91.1%. When the entrance of water was stopped, the bioactivityof the biofilm can be recovered by aeration. Under the natural environment, the bacterial accompanied and survived with NY3 in bacteria biofilmmainly includedBacillus, Pseudomonas and Rhodococcus strain. Two cultured strains were isolated and identified through the 16SrRNAsequence, whic