氮肥对水稻再生芽萌发生长过程蛋白质表达的影响-中国生态农业学报.DOC

促芽肥对水稻再生芽萌发生长过程蛋白质表达的影响 陈鸿飞1,2 张志兴1,2 林文雄1,2** (1. 福建农林大学生命科学学院福州350002; 2. 福建农林大学农业生态研究所 福州 350002) 摘 要 采用比较蛋白质组学的方法和技术, 并结合相关生理指标测定, 探讨了促芽肥对水稻再生芽萌发生长过程中蛋白质表达及相应生理特性的影响。共获得了20个在不同促芽肥下再生芽萌发生长过程出现差异表达的蛋白质点, 涉及再生芽萌发生长过程的能量代谢、生长调控和抗逆响应等, 在其他栽培措施一致的情况下, 施用促芽肥能有效地降低再生芽萌发生长过程中能量代谢相关蛋白的下调幅度, 提高光合相关蛋白的表达, 从而使再生芽具有相对较强的能量合成能力和较强光合自养能力, 更好地满足再生芽分化生长对能量和物质的需求; 还能相对降低抑制再生芽萌发生长相关蛋白的表达, 提高促进再生芽分化过程细胞分裂和伸长相关蛋白及抗性相关蛋白的表达, 促进再生芽的萌发生长, 增强再生芽抵御外界生物和非生物胁迫的能力, 提高对各种不良环境的适应性, 因而其倒二至倒五各节位再生芽的活芽率和芽长极显著高于未施用促芽肥处理, 从而显著提高了再生季的单位面积有效穗数、结实率和产量, 实现了再生季的高产增产。 关键词 水稻 再生芽 促芽肥 蛋白 产量 中图分类号：S511.01 文献标识码：A Effects of nitrogen application for bud development on protein expression of ratooning buds CHEN Hongfei1,2, ZHANG Zhixing1,2, LIN Wenxiong1,2** (1.School of Life Sciences, Fujian Agricultural and Forestry University, Fuzhou 35002, China; 2.Institute of Agricultural Ecology, Fujian Agricultural and Forestry University, Fuzhou 350002, China) Abstract Ratooning rice with large improvements in potential yield, has become a farming system on raise multiple crop index. For ratooning rice yield is directly related to the rate of ratooning buds germinating into spike, enhancing the ratooning buds germination is the key of high yield in ratooning rice. Numerous studies have shown that nitrogen application for bud development could strongly promote the ratooning buds germination, and many reports on eco-physiology of which were performed. However, the molecular mechanism of nitrogen application for bud development promoting the ratooning buds germination is now unclear. This paper studied the effects of nitrogen application for bud development on protein expression of ratooning buds and the corresponding physiological characteristics by using comparative proteomics combined with related physiological indicators. A total of 20 differentially expressed proteins were detected in different fertilizer treatments by the 2D maps of ratooning buds proteome. And, these proteins were grouped into three expre