宁夏温室瓜菜白粉病菌鉴定及病害流行预测模型构建.PDFVIP

  • 11
  • 0
  • 约3.23万字
  • 约 8页
  • 2017-12-23 发布于天津
  • 举报

宁夏温室瓜菜白粉病菌鉴定及病害流行预测模型构建.PDF

宁夏温室瓜菜白粉病菌鉴定及病害流行预测模型构建.PDF

植物保护学报Journal of Plant Protection ,2017 ,44 (5):788-795 DOI :10.13802/ki.zwbhxb.2017.2016053 宁夏温室瓜菜白粉病菌鉴定及病害流行 预测模型构建 1 1 2* 1* 李磊福 孙秋玉 史 娟 马占鸿 (1. 中国农业大学植物保护学院植物病理学系,北京 100193;2. 宁夏大学农学院,银川750001 ) 摘要:为明确宁夏回族自治区温室瓜菜白粉病菌的分类地位,对采自该地区温室的南瓜、黄瓜和甜 瓜上的白粉病菌基于ITS 序列分析进行分子鉴定;利用孢子捕捉器对温室中甜瓜白粉病菌的孢子 量进行监测,分析环境因子、孢子量和病情指数之间的关系,并采用逐步回归分析法构建温室甜瓜 白粉病的流行预测模型。结果表明,基于ITS 序列的分子鉴定结果,3 种瓜菜白粉病的病原菌均为 单囊壳白粉菌Podosphaera xanthii 。发病期间,每日温室中甜瓜白粉病菌的孢子量在12: 00— 16: 00 时段最多,占24 h 内总孢子量的34%~81%,20: 00—08: 00 时段最少;白粉病菌孢子的释放与 光照强度有关,相关系数为0.602 。第t 天的病情指数与标准累积温度、标准累积湿度、t-4 d 前 08: 00—12: 00 时段的累积孢子量、第t-4 天16: 00—20: 00 时段的孢子量均具有显著的相关性,相 关系数分别为0.935、0.938、0.956 和0.921。以标准累积湿度和第t-4 天16: 00—20: 00 时段的孢子 量为预测变量构建了温室甜瓜白粉病流行预测模型,决定系数为0.962 ,表明该模型具有较好的实 际应用价值。 关键词:瓜菜白粉病;ITS 序列;孢子量;环境因子;预测模型 Pathogen identification and forecasting model construction of cucurbit powdery mildew in greenhouse in Ningxia Li Leifu1 Sun Qiuyu1 Shi Juan2* Ma Zhanhong1* (1. Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing 100193, China; 2. College of Agriculture, Ningxia University, Yinchuan 750001, Ningxia Hui Autonomous Region, China) Abstract: In order to determine the pathogen of cucurbit powdery mildew in Ningxia, the sample of cu- curbit powdery mildew were collected from pumpkin, cucumber and melon leaves to amplify their ITS sequences and construct phylogenetic trees using a neighbor- joining method. Meanwhile, spore traps were used to monitor the number of airborne conidia of melon powdery mildew in greenhouse. The rela- tionship among environmental factors, the number of airborne conidia and the disease index

文档评论(0)

1亿VIP精品文档

相关文档