大孔树脂分离纯化核桃青皮总黄酮的研究-生物质化学工程.PDFVIP

第42卷第3期 生摇 物摇 质摇 化摇 学摇 工摇 程 Vol.42 No.3 2008年5月 Biomass Chemical Engineering May2008 大孔树脂分离纯化核桃青皮总黄酮的研究 翟梅枝,郭 琪, 贾彩霞,张新华 ( 西北农林科技大学林学院, 陕西 杨凌 712100) 摘摇 要:以总黄酮回收率为考察指标,研究了大孔树脂分离纯化核桃青皮总黄酮的工艺。 结果表明:D101型树脂对核桃 青皮总黄酮有较好的吸附分离性能,是分离纯化核桃青皮总黄酮的适宜大孔树脂;最佳工艺条件为:上柱总黄酮与干树 脂质量比为1 颐12,上样液质量浓度可在3.0875~6.175g/ L范围内,pH值为5,6BV(1BV=23.7mL)的水洗后用5BV 的 70%乙醇洗脱。 经D101处理后的核桃青皮总黄酮回收率在60%,纯度可达80%以上。 该工艺简便,能有效分离纯化核 桃青皮黄酮类化合物。 关键词:核桃青皮;总黄酮;大孔吸附树脂;分离纯化 中图分类号:TQ91;TQ425摇 摇 摇 摇 摇 摇 摇 文献标识码:A摇 摇 摇 摇 摇 摇 摇 文章编号:1673-5854(2008)03-0021-05 Study on the Separation and Purification of Flavonoids from Walnut Peel with Macroporous Resin ZHAI Mei鄄zhi,GUO Qi,JIA Cai鄄xia,ZHANG Xin鄄hua ( College of forest,Northwest Agriculture and Forestry of University,Yangling712100,China) Abstract:A methodfor separation and purification of flavonoids from walnut peel with macroporous resin was studied with the recovery rateofflavonoidsasindexes. Theresultsareasflollows:among sixtypesof macroporousadsorptionresinchoosed,type macroporous resin D101 is the best for separation and purification. The optimum process condition was the loaded amount of flavonoids todryresin:1 颐12,thesampleconcentration:3.0875-6.175g/ L,pH:5. Afterelutedwith6BVofdistilledwaterand 5BV of70% ethanol,theyield of flavonoidswas above60% and product purity was above80%. The process is simple andthe flavonoids of walnut peel can be separated and purified effectively. Key words:walnut peel;flavonoids;macroporous adsorption resin;separation and purification 摇 摇 核桃(Juglans regia Linn.)是核桃属植物中 指标,对大孔树脂分离纯化核桃青皮中的总黄酮 经济价值最高的一种,在我国栽培历史悠久,资源 进行了研究。 丰富,到 2004 年,我国核桃树的栽培面积约 2 1摇 实 验 91.75hm ,2亿株左右,面积和株数都居世界第 一位,年产坚果 27.4246 万吨,核桃青皮 35 万