王不留行主要成分对小鼠乳腺上皮细胞增殖及β-酪蛋白表达的-生物通.docVIP

王不留行主要成分对小鼠乳腺上皮细胞增殖及 β-酪蛋白表达的影响 秦 君， （东北农业大学乳品科学教育部重点实验室，哈尔滨 150030Effect of Main Components from Semen Vaccariae on Multiplication and Expression of Beta-Casein of Mouse Mammary Epithelial Cell in vitro QIN Jun, LI Qing-zhang, GAO Xue-jun (Key Laboratory of Dairy Science Ministry of Education, Northeast Agricultural University, Harbin 150030) Abstract:【Objective】The objective of this test is to find one or several effective components in promoting lactating of Semen accariae, so as to provide scientific basis for the research and exploitation of galactopoiesis with Chinese traditional medicine.【Method】he extracting methods of the three main components from Semen vaccariae, saponin, flavonoid glycoside and coumarin, and the identification by using chemical methods and chromatographic methods were studied. Add these components to cell cultural medium at different concentrations. Then their effects on multiplication of mouse mammary epithelial cells were detected by using the mothod MTT and the expression of beta-casein of mouse mammary epithelial cells was detected using real-time PCR. Furthermore, their mechanism was also studied. The expression of cyclin D1 and phosphatic stat5 was detected using laser scanning confocal microscope. Finally, the function of the effective component was validated through animal experiment.【Result】The result indicated that the components of saponin could restrain the multiplication of mouse mammary epithelial cells (P＜0.05, which had no influence on the expression of beta-casein (P＞0.05. The components of coumarin had no influence on both the multiplication of mouse mammary epithelial cells and the expression of beta-casein (P＞0.05. The components of flavonoid glycoside could promote the lactation of mouse. They could promote the multiplication of mouse mammary epithelial cells by activating the expression of cyclin D1 and regulate the expression of beta-casein by activating Jak-stat5.【Conclusion】avonoid glycoside is o