响应面法优化火炭母中总黄酮的提取工艺研究.pdfVIP

目 录 摘要 Ⅰ- Ⅱ 1 前言1 2 材料与仪器3 2.1 试剂与试药3 2.2 仪器3 3 方法4 3.1 芦丁对照品标准曲线的建立4 3.2 方法学考察4 3.3 单因素考察方法7 3.4 结果10 4 讨论14 4.1 提取条件分 14 5结论16 参考文献17 综 述19 参考文献23 致 谢25 响应面法优化火炭母总黄酮提取工艺研究 摘要:目的 采用响应面法优化火炭母总黄酮的提取工艺。方法 以火炭母为试材，以乙醇溶液为提取溶剂，在单因素实验的基础上，采用响应Bo x- Behnken进行实验设计，对火炭母中黄酮类物质的提取条件进行优化，考察了料 液比，提取时间，乙醇浓度对提取率的影响。结果 黄酮类物质的最佳提取条件为提取剂67.21% 乙醇，料液比1:79.09，提取时间2.03 h ，提取率为20.26mg/g。结论 该工艺经试验含量测定方法简便、准确、重复性好，提取工艺经济、合理，值得进 一步推广，对进一步的工艺开发有 好的参考价值。 关键词: 火炭母；总黄酮；响应面法 Optimization Extraction Conditions of Total Flavonoids form Polygonum chinense by RSM Abstract: Objective Optimization extraction conditions of total flavonoids form Polygonum chinense by RSM. Methods With Polygonum chinense as material and ethanol as solvent.the extraction of total flavonoids from Polygonum chinense were optimized by response surface methodology based on Box-Behnken.The research had investigated the influence of some factors on extraction effect of total flavonoids which included ratio of material and ethanol, extraction time, and liquid. Results The optimum extraction conditions were as follows extraction was 67.21%ethanol, ratio of material and liquid was 1:79.09, extraction time was 2.03h, and the extraction rate of flavonoid was20.26mg/g.Conclusion The content determination method is simple, accurate and reproducible. Extraction which is technology, reasonable and economy worths of further promotion and to the further development ,it has good reference value. Keyword: Polygonum chinense; flavonoid; response surface methodology I II 1 前言 火炭母是蓼科蓼属植物火炭母（Polygonum shinese L .）的干燥全草[1- 2] ，《植物名实图考》：“其子青黑如炭。”[3]故名。别名火炭毛，乌炭子，乌 饭草， 山荞麦草，火炭星、鹊糖梅，赤地利、乌饭藤、水沙柑子、鸪鹚饭、水退痧，红梅子 [4] 叶、 饭草、大叶沙滩子，老鼠蔗等 ，生于水边、山谷、湿地，分布于分布于浙江 、江西、福建、台湾、湖北、湖南、广东、海南、广西、四川、贵州、云南、西藏等地