卵形孢球托霉NL-15GongronellabutleriNL-15-应用与环境生物学报.DOC

卵形孢球托霉NL-15（Gongronella butleri NL-15）对石灰岩侵蚀机制的研究* 王广林1,2 邓 辉1 聂 丽1 吴雁雯2 张金池2** 1 皖西学院分析测试中心 六安 237012 2 南京林业大学森林资源与环境学院 南京 210037 摘 要 为了探讨微生物对石灰岩侵蚀的作用机制，选择从岩石表面分离纯化出的一株优势、高效石灰岩侵蚀菌Gongronella butleri NL-15，采用岩石粉发酵培养，研究菌株发酵不同时期发酵液的pH、主要离子浓度、有机酸含量及其岩石颗粒的表面形貌特征。结果表明：菌株发酵液的pH值与Ca、Mg离子含量显示显著的负相关性，对照的pH值为6.87，而15 d发酵液的pH值降低到5.12；对照的Ca离子含量为38.96 mg/L、Mg离子为10.85 mg/L，而15 d发酵液的Ca、Mg离子分别升高到367.56和76.16 mg/L。总有机酸和乳酸的含量随着发酵时间的增加而增多，2、5、9、15 d的发酵液中总有机酸含量分别为3.43、5.40、6.63和7.26 mg/mL,乳酸含量分别是1.79、2.85、5.16和5.04 mg/mL。菌丝体包裹岩石颗粒，插入其空穴，崩解岩石颗粒。因此，菌株产生有机酸特别是乳酸导致岩石疏松化以及菌丝体生长穿插入疏松化的岩石内部，是其侵蚀石灰岩释放出Ca、Mg等离子的主要因素。图3表2参19 关键词 卵形孢球托霉；发酵；岩石侵蚀；客土喷播 CLC Q939.99 Limestone corrosion mechanism of the highly-efficient limestone corrosion strain Gongronella butleri NL-15* WANG Guanglin1,2, DENG Hui1, NIE Li1, WU Yanwen2, ZHNAG Jinchi2** 1 Instrumental Analysis Center, West Anhui University, Liu’an 237012, China 2 College of Forestry Resources and Environment, Nanjing Forestry University, Nanjing 210037, China Abstract In order to investigate the limestone corrosion mechanism of microorganisms, the dominant and highly-effect limestone corrosion strain Gongronella butleri NL-15 isolated and purified from the microorganisms on the rock surface was selected in the study. ICP analysis Ca, Mg, K and P element concentration and precise pH instrument analysis pH value in the different time fermentation broth of the strain were studied. At the same time, HPLC analysis of the?organic?acids ( Critic acid, Succnic acid, Lactic acid, Fumaric acid, Acetic acid and Propanoic acid ) and the scanning?electron?microscope feature of rock particles in the different time of the fermentation broth from the strain were also carried out. The results showed that Ca2+ and Mg2+ concentration in the different time fermentation broth of the strain had negative relativity to pH 收稿日期 Received： 接受日期 Accepted： * 安徽省教育厅自然科学研究重点项目（KJ2016A744）和第五十批中国博士后科学基金面上资助项目（2011M500931）资助 Supported by the Key