生命的奥秘9 重建身体.ppt

* * * * * 成年动物的许多组织和器官，如表皮和造血系统，具有修复和再生的能力。成体干细胞在其中起关键的作用。 Nuclear reprogramming to a pluripotent state by three approachesShinya Yamanaka Helen M. BlauNATURE|Vol 465|10 June 2010|doi:10.1038/nature09229 * * * * Clinical photographs of a 21-year-old patient who underwent repeat limbal biopsy in the right eye and repeat autologous cultivated limbal epithelial transplantation in the left eye for limbal stem cell deficiency following ocular surface burns. (Top left) One day after repeat limbal biopsy, performed nasal to the site of original biopsy, a 3 4-mm limbal and conjunctival epithelial defect is seen. (Top right) One week after the biopsy, the limbal site is completely epithelialized with a small 2 1-mm epithelial defect on the conjunctiva. (Bottom right) Ten days after the repeat biopsy, a small linear epithelial defect is seen over the bulbar conjunctiva with pooling of fluorescein dye over the epithelialized donor site. (Bottom left) Two weeks (on the day of transplantation in the left eye) after repeat biopsy, the ocular surface has completely epithelialized. Clinical Outcomes of Repeat Autologous Cultivated Limbal Epithelial Transplantation for Ocular Surface Burns SAYAN SAYAN BASU, MD HASNAT ALI, AND VIRENDER S. SANGWAN,2011 N Engl J Med 2010; 363:147-155 Limbal Stem-Cell Therapy and Long-Term Corneal Regeneration Paolo Rama Nature. 2016 Mar 17;531(7594):323-8. doi: 10.1038/nature17181. Epub 2016 Mar 9. Lens regeneration using endogenous stem cells with gain of visual function. a, Pax6-directed GFP was expressed in mouse LEC nuclei at post-natal days P1, P14 and P30; a sagittal section of a P0-3.9-GFPcre mouse lens is shown. Blue and green represent DAPI and GFP, respectively. b, Lineage tracing of Pax6+ LECs in ROSAmTmG; P0-3.9-GFPcre mice at P1, P14 and P30 reveals that lens fibre cells express membrane GFP fluorescence; hence, PAX6+ LECs were able to generate lens fibre cells. c, As an additional control, the ROSAmTmG allele alone exhibits Tomat