普洱茶对主要发炎和抗炎细胞因子的影响.pdfVIP

428 2008, Vol. 29, No. 07 食品科学 ※营养卫生 于立芹，庞广昌* ，李珍珍 (天津商业大学生物技术与食品科学学院，天津 300134) ：目的：观察及评价不同年份普洱茶通过不同给药途径对小鼠血清中IL-6 、IFN- γ、IL-1 β、TNF- α、 IL-10 、IL-4 水平的影响，进而研究普洱茶对免疫系统的调节作用。方法：通过灌胃、腹腔注射、尾静脉注射 三种途径分别给小鼠当年普洱茶茶和30 年普洱茶，灌胃组在给药4h 后眼球取血，腹腔注射和尾静脉注射组在给药 3h 后眼球取血，分离血清，采用ELISA 法检测血清中IL-6 、IFN- γ、IL-1 β、TNF- α、IL-10 、IL-4 的含量。 结果：实验组三种给药途径对小鼠血清中IL-6 、IFN- γ、IL-1 β、TNF- α、IL-10 、IL-4 与对照组相比都有显 著性差异；给药组小鼠血清中IL-6 、IFN- γ、IL-10 、IL-4 含量明显高于相应的对照组，TNF- α、IL-1 β含量 显著低于对照组。三种给药途径中，腹腔注射普洱茶对细胞因子的影响最为显著。结论：不同年代的普洱茶对细 胞因子的影响效果不同，普洱茶的免疫调节等一系列生物功能可能是通过对肠粘膜系统中免疫细胞产生的细胞因子 的影响发挥的。 ：普洱茶；细胞因子；Balb/c 小鼠 Effects of Puer Tea on Production of Pro- and Anti- inflammatory Cytokines YU Li-qin ，PANG Guang-chang* ，LI Zhen-zhen (College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China) Abstract ：Objective: To explore and evaluate the effects of Puer tea on the levels of IL-6,IFN- γ,IL-1 β,TNF- α, IL-10 and IL-4 in serum of mice，and then study the regulative effect of Puer tea on immune system. Methods: Puer tea was adminis- trated to mice by oral administration, intraperitoneal injection and intravenous injection, which was conserved for 1 year and 30 years respectively. For oral administration group, serum was separated from eyeball at 4 h after administration, for intraperitoneal injection and intravenous injection groups at 3 h after administration. The contents of IL-6 ，IFN- γ，IL-1 β，TNF- α， IL-10 and IL-4 were examined by ELISA assay. Results: The contents of IL-6, IFN- γ, IL-10 and IL-4 in mice serum of experiment group significantly increase compared with contents of those in mice serum of corresponding control group, but the cont