DHA含量测定-英文版.docVIP

Weishi Biological Technology Co., Ltd. Zhejiang The determination of the content of the DHA Principle Fatty acids in oils and fats unter the boron (tri)fluoride and methanol and sodium hydroxide , as a fatty acid methyl ester, the fatty acid methyl ester’s respondence on gas chromatograph hydrogen flame ionization detector linearly related with its existent amount. The content of variety of fatty acids can be calculated by the content of a variety of fatty acid methyl ester on chromatography. Application Applicable to the determination of conventional content of DHA oil or powder. Sample volume Sample about 2g. Apparatus 4.1 Volumetric flask of 25ml 4.2 Transfer pipet of 10ml 4.3 Gas chromatography equipment and operating conditions Column Oven Initial temperature：90℃（开） Maximum temperature: 300℃ The initial time： 1.00 min Balance time: 0.20 min Temperature gradient # Career The final temperature The final time Operating temperature after:50℃ 1 9.00 240 5.00 Operating time after: 0.00min 2 3.00 250 0.00 Operating time: 26.00 min 3 0.0(关) Forward injection port (streaming / non-streaming) mode: streaming, 1:100 ，initial temperature:250℃(开) chromatographic column Capillary column,specification:DB-23 122-2332 Maximum temperature:300 Length:30.0m Diameter:250.00um Film thickness:0.25um Detector (FID) Temperature ：300℃ flow rate of hydrogen 30ml/min Make up flow 29ml/min flow rate of air 43ml/min flow rate of carrier gas 1.33ml/min Reagent 5.1 Taka-Diastase，SIGMA. 5.2 Anhydrous ethanol, AR, Bengbu chemical reagents factory. 5.3 Anhydrous ether, AR , Tianjin Guangcheng Chemical Reagent Co., Ltd. 5.4 Petroleum ether, AR, Tian jin (Hong Kong) xingtong fine Chemical Co., Ltd. 5.5 0.5M sodium hydroxide solution of methanol: get sodium hydroxide about 2g, put it in 100ml absolute methanol. 5.6 Boron (tri)fluoride methanol solution: The boron (tri)fluoride or boron (tri)fluoride di