甘草附子汤和术附汤肠内生物转化的电喷雾质谱.pdfVIP

2011 年第69 卷 化 学 学 报 Vol. 69, 2011 第11 期, 1368～1374 ACTA CHIMICA SINICA No. 11, 1368～1374 ·研究论文· “甘草附子汤”和“术附汤”肠内生物转化的电喷雾质谱研究 王曦烨a,b 皮子凤a 宋凤瑞a 刘志强a 刘淑莹*,a (a 中国科学院长春应用化学研究所 长春 130022) (b 中国科学院研究生院 北京 100039) 摘要 以电喷雾质谱法作为研究方法, 以内标化合物为切入点, 对复方中双酯型、单酯型及脂型生物碱的生物转化进 行了深入研究, 建立了电喷雾质谱对代谢前后生物碱成分的半定量分析方法. 分析了中药复方甘草附子汤和术附汤经 大鼠肠内菌群代谢主要生物碱的含量变化, 研究结果表明, 配伍中药甘草和白术可以有效地降低共煎液中双酯型生物 碱含量, 在代谢过程中, 大鼠肠内菌群能够进一步将复方中双酯型生物碱转化为脂型生物碱, 从而达到中药配伍的减 毒增效目的. 关键词 甘草附子汤; 术附汤; 肠内菌代谢; 电喷雾质谱 Studies on the Biotransformation of Licorice and Aconite Accessory Root Decoction and Atractylodes Macrocephala and Aconite Accessory Root Decoction under Rat Intestinal Bacteria Wang, Xiyea,b Pi, Zifenga Song, Fengruia Liu, Zhiqianga Liu, Shuying*,a a ( Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022) b ( Graduate School of the Chinese Academy of Science, Beijing 100039) Abstract The biotransformation of diester-diterpenoid alkaloids, monoester-diterpenoid alkaloids and lipo-alkaloids in compound recipe was studied by electrospray ionization tandem mass spectrometry and a method of semiquantitative ESI-MS was established for analysis of aconitum alkaloids before and after me- tabolism. The content changes in the process of metabolism of aconitum alkaloids in Licorice and Aconite Accessory Root Decoction and Atrac