DBS英文版流程示意.pdfVIP

ACTN Laboratory Technologist Committee Version 1.1 ACTN Dried Blood Spots Procedure 11 March 2009 DRIED BLOOD SPOTS CARD COLLECTION, PROCESSING, AND STORAGE PROCEDURES 1. Rational 1.1 Dried blood spots (DBS) have been used for years for HIV-1 RNA, DNA, Genotyping, and other diagnostic assays. Dried blood spots can be used as an easy and inexpensive means for the collection and storage of peripheral blood specimens from infants, children and adults in settings where collection and storage of plasma is not optimal. Dried blood spots use a small amount of peripheral blood on protein saver filter paper cards from infant heel pricks, or finger sticks from adults and require less skill and supplies than venous blood draws. The cost and difficulties of cold- chain shipping of plasma samples is greatly reduced by the use of dried blood spots and they can be shipped as non-dangerous goods. 2. REAGENTS AND MATERIALS 2.1 Materials for Heel Stick Method 2.1.1 Unistick 2 device (Fisher Scientific #22-0227) 2.1.2 Alcohol swab 2.1.3 Dry sterile gauze pad 2.1.4 Whatman Protein Saver Card #903 (Whatman Fisher Scientific # NC9307519) 2.1.5 Gas impermeable storage bag (Fisher Scientific# NC9307519) 2.1.6 Desiccant pack (WhatmanFisher Scientific# 2.1.7 Humidity indicator Cards (Multisorb Des Manufacture # MS200032 Fisher Scientific # NC9511648) 2.1.8 Whatman card drying rack, (VWR catalogue # 89015-592) 2.1.9 Gloves preferably powder free 2.1.10 Water proof marker 2.2 Materials for Veno