急性坏死性胰腺炎论文：组织因子途径抑制物与大鼠急性坏死性胰腺炎发生发展的关系.docVIP

急性坏死性胰腺炎论文：组织因子途径抑制物与大鼠急性坏死性胰腺炎发生发展的关系 【中文摘要】探讨组织因子途径抑制物（简称TFPI）在急性坏死性胰腺炎(简称ANP)发病中的变化及作用。方法1.将60只Wister大鼠，随机分为假手术组、造模组和治疗组，每组20只，采用5%牛磺胆酸钠胰胆管逆行注入制作ANP模型。2.治疗组于术后2、5、8、11h分次背部皮下注射生长抑素生理盐水溶液100μg / L，剂量为15μg/kg；假手术组，造模组均注射等体积生理盐水。3.术后6、12h分批处死大鼠，留取血样及胰腺标本，检测不同时间点血清Ca浓度和TFPI值，并对胰腺组织进行病理学观察。结果1、血清TFPI检测结果：同一时间，造模组与假手术组相比，TFPI值显著升高(P 0. 01)；治疗组与造模组相比，TFPI值明显降低( P 0. 05)。组内比较，随时间延长，造模组TFPI表达逐渐升高；治疗组TFPI表达逐渐降低，各组6h、12h之间比较有显著差异( P 0. 05)。2、血清Ca检测结果：同一时间，造模组与假手术组相比，血Ca明显降低( P 0. 05)；治疗组比造模组，血Ca升高( P 0. 05)。组内比较，随时间延长，造模组血Ca逐渐降低；治疗组血Ca升高，各组6h、12h之间比较有显著差异( P 0. 05)。3、病理学结果：同一时间，造模组与假手术组相比胰腺组织病理损害严重；组内比较，随时间延长，假手术组无明显变化，而造模组随时间延长胰腺组织损伤加重。与造模组相比，治疗组胰腺组织损伤程度较轻，治疗组组内比较，随治疗时间延长，损害减轻程度逐渐减轻。结论TFPI与胰腺组织损伤呈正相关，TFPI可以减轻胰腺细胞的损伤和坏死，可能阻止多器官功能衰竭的发生和进展。 【英文摘要】The effect of tissue factor pathway inhibitor in rat with acute necrotizingpancreatitisTo investigate the changes and effects of tissue factor pathway inhibitor（TFPI）in rat withacute necrotizing pancreatitis.MethodsSixty adult Wistar rats were randomly divided into three groups: Sham operation group,Model group and Treat group；Model group was treated by normal saline, and treat group wastreated by Somatostatin group; ANP rat model were established by retrograde injection ofsodium taurocholate into the biliopancreatic .Compare the three groups’ concentration of serumCa、TFPI and Pancreatic pathological section.ResultsCompared with the Sham operation group, TFPI in Treat and Model group were assignificantly increased(P 0.05); Ca in Treat and Model group of serum were significantlydecreased(P 0.05). Compared with the Model group, TFPI in Treat group was significantlydecreased(P 0.05), and Ca in Treat group was significantly increased(P 0.05). The degree ofpancreatic tissue sections lesion in Treat group was significantly decreased at 6、12 hour.ConclusionTFPI and pancreatic tissue injury have positive correlation. TFPI can reduce cellularinjury and necrosis of the pancreas and prevent the occurrence of MODS progress. 【关键词】急性坏死性胰腺炎 组织途