关节炎论文：脂联素及其受体在原发性痛风患者外周血的表达及临床意义.docVIP

关节炎论文：脂联素及其受体在原发性痛风患者外周血的表达及临床意义 【中文摘要】检测脂联素（Adiponectin, ADP）及脂联素受体1,2(Adiponectinreceptor1,2；ADR1,ADR2)在原发性痛风患者（GA）及健康对照者（NC）外周血的表达水平，并比较其差异性，探讨脂联素在痛风炎症反应中可能的作用。方法:（1）详细收集88例原发性痛风患者和80例健康对照者的临床资料，并分离外周血单个核细胞（peripheral blood monouclear cell,PBMC）。（2）用酶联免疫吸附试验（enzyme linked immunosorbent assay,ELISA）检测ADP在原发性痛风患者及健康对照者的血浆水平，并比较两组间的表达差异性；分析ADP与痛风患者临床及实验室指标的相关性。（3）用实时荧光定量聚合酶链反应（real-time quantitative polymerase chainreaction, RT-qPCR）检测ADR1,ADR2在原发性痛风患者及健康对照者PBMC的基因水平，比较ADR1 mRNA和ADR2 mRNA水平在PBMC中的表达差异性；分析ADR1和ADR2基因与痛风患者临床及实验室指标的相关性。结果：（1）GA组血浆ADP含量显著低于NC组（6.32±6.72μg/ml vs8.36±6.37μg/ml，P0.05）。（2）ADR1、ADR2 mRNA表达量在GA组则显著高于NC组（ADR1：0.0858±0.0784 vs 0.0458±0.0337, ADR2：0.0122±0.0164 vs 0.0054±0.0024；P均0.05）。(3)Spearman相关分析发现痛风患者ADP含量与淋巴细胞绝对值（LY）、血尿酸（UA）均呈显著负相关（r=-0.32，r=-0.36，P均0.05），与血沉（SR）和低密度脂蛋白（LDL）均呈显著正相关（r=0.31，r=0.39，P均0.05)；痛风患者ADR1mRNA表达量与甘油三酯（TG）呈显著负相关(r=-0.43，P0.05)，与SR、C反应蛋白（CRP）则呈显著正相关（r=0.45，r=0.57，P均0.05），ADR2mRNA表达量与血糖（GLU）和UA均呈显著负相关（r=-0.50，r=-0.59，P均0.05）。（4）多元逐步回归分析发现痛风患者ADP水平受年龄因素影响，ADR1 mRNA受UA影响（P=0.006），ADR2 mRNA受TG（P=0.0005）、UA（P=0.02）、LDL（P=0.03）及GR（P=0.005）影响。结论:ADP及其受体表达异常可能参与了原发性痛风的发生发展，可能与痛风的炎症、免疫及代谢过程密切相关。 【英文摘要】: To study relevant molecular expression of adiponection(ADP) and its receptors in peripheral blood from patients with primary goutyarthritis (GA) and healthy individuals; to explore the role of ADP in thepathogenesis of GA.Methods: (1)88 GA patients and 80 healthy controls (NC) were enrolledinto the study. The clinical data of primary gouty arthritis patients and healthyindividuals were collected. PBMC were isolated from blood in vitro. (2)Thelevels of ADP in plasma of primary gouty arthritis patients and healthyindividuals using enzyme linked immunosorbent assay (ELISA). (3)Real timequantitative polymerase chain reaction (RT-qPCR) was employed to study theexpression of ADR1 mRNA and ADR2 mRNA in PBMC from GA and NC,and the differences of genes expression in two groups were compared,Spearman correlation and multiple regression a