曾益新院士 ：Cancer Stem Cells or Stem-like Cancer Cells .pptVIP

Cancer Stem Cells or Stem-likeCancer Cells in NPC? Yi-Xin Zeng Sun Yat-Sen University Cancer Center A frequently asked question in clinical practice: How can we predict the outcomes of NPC patients (Stage II/III) without visible tumors after regular therapeutics? In other words: What causes cancer recurrence or metastasis? Cancer stem cells (CSCs) Self-renewal Differentiation Chemoresistant Radioresistant Invasive metastatic CSCs from different types of cancer NPC cells are a population with heterogeneity There is a sub-group of cell (SP) exhibited stem-cell-like characteristics, such as proliferation, self-renewal and differentiation, strong tumorigenesis ability, resistant to chemotherapy. Origins of CSC? Transformation of adult stem cells Genomic instability of cancerous cells? The chromosome numbers varied from 17 to 107 The size of nuclei differs from 5 to 58 mm Loss function of p53 (genome guardian) Mutations of Myc, etc A series of structurally abnormal chromosomes detected by CGH, LOH or FISH “Stem-like” is changeable phenotype Genomic instability results in formation of stem-like cancer cells Cancer-initiating cells: CSC (transformed tissue stem cells), transformation of progenitor or differentiated cells Stem-like cancer cells: cause of cancer recurrence or metastasis Thank you! * * Liang et al., Fig. 1 (Wicha et al., 2006) (Bonnet et al., 1997) Identification of the first CSC ABCB5+ Melanoma CD34+ Skin cancer (Mus muscular) CD133+; CD90+; EpCAM Hepatocellular carcinoma ABCG2 (Side Population) Nasopharyngeal carcinoma CD133+; CD15+ Glioma CD133+; Lgr5+ Colon cancer CD44+CD24-/low; Breast cancer CD34+CD38-Thy-1-; CD47+ Acute myeloid leukemia Marker(s) of CSCs Cancer (Forbes et al., 2006) The principle of side-population sorting (Wang et al., Cancer Research 2007) Summary Genomic instability of NPC cells Chromosome loss and gain Chromosome 10 Chromosome 11 Chromosome 14 Chromosome 17 Chromosome 20