il-1β抑制大鼠离体幽门括约肌自发收缩的影响因素.docVIP

精品论文 参考文献 IL-1β抑制大鼠离体幽门括约肌自发收缩的影响因素 济源职业技术学院护理系 河南 济源 459000 摘要： 目的 观察IL-1beta;抑制大鼠离体幽门括约肌自发收缩的影响因素，初步探讨其作用机制，为IL-1beta;在平滑肌舒张方面的作用机制作进一步补充，为其临床应用提供实验室的依据。方法 成年SD大鼠（雌雄不拘）随机分为正常组、IL-1beta;组、格列苯脲组、吲哚美辛组。采用离体组织灌流的方法，用RM6240C生物信号采集系统观察IL-1beta;对大鼠离体幽门括约肌自发收缩作用的影响；通过应用不同信号途径的特异性阻断剂，探讨IL-1beta;的作用机制；所有数据用SPSS17.0统计软件进行分析。结果 1．IL-1beta;组：IL-1beta;（终浓度为2ng/ml、20ng/ml、200ng/ml）能抑制大鼠离体幽门括约肌自发收缩的振幅、频率和张力（P＜0.05，n=9）。2．格列苯脲（5mu;M）对IL-1beta;抑制大鼠离体幽门括约肌的自发收缩作用无影响（P＞0.05，n=6）。3．吲哚美辛（10mu;M）能部分抵消IL-1beta;对大鼠离体幽门括约肌自发收缩的抑制作用，与IL-1beta;单独作用相比，振幅、频率和张力均较高（P＜0.05，n=6）。小结 IL-1beta;能抑制大鼠离体幽门括约肌的自发收缩，其作用可被吲哚美辛部分阻断，而不受格列苯脲影响，说明其机制很可能与前列环素有关，而与KATP通道无关。 关键词： IL-1beta; Ca2+ 幽门括约肌 KATP 前列环素 【中图分类号】R473【文献标识码】A【文章编号】1001-5213(2016)08-0317-02 Influence factor of IL-1beta; on spontaneous contraction of isolated ratsrsquo; pyloric sphincter Fan Shuangli （Nursing department, Jiyuan Vocational and Technical College , Henan Jiyuan,459000） Abstract Objective This study was planned to investigate the effect and mechanism of IL-1beta; on the spontaneous contractions of isolated ratsrsquo; pyloric sphincter in order to replenish the pharmacological effect of IL-1beta;.Methods Male or female Sprague-Dawley rats were divided into four groups randomly: normal group, IL-1beta; group, indomethacin group and glibenclamide group, then killed by cervical dislocation to excise the pyloric sphincter. Perfusion of isolated rat pyloric sphincter was used to evaluate the effect of each group. Results 1. IL-1beta; group:Addition of the IL-1beta; induced an inhibition of the spontaneous contractions. The amplitude, frequency and tension were decreased.2. Pretreatment of the tissues with glyburide (5mu;M) was without significant effects on the spontaneous contractions responses to IL-1beta; (Pgt; 0.05, n = 6).3. Pretreatment of the tissues with indomethacin (10mu;M) attenuated the inhibitory effects of IL-1beta;. The amplitude, frequency and tension were increased compared with indomethacin