下调 NF-KB／p65的表达对小鼠移植瘤细胞Bcl-2和Bax的影响.docVIP

精品论文 参考文献 下调 NF-KB／p65的表达对小鼠移植瘤细胞Bcl-2和Bax的影响 1中国医科大学附属盛京医院第二肿瘤科 沈阳 110000；2.中国医科大学附属盛京医院第四肿瘤科 沈阳 110000 【摘 要】目的：研究利用p65siRNA下调NF-kappa;B p65表达后，对小鼠Lewis肺癌移植瘤细胞组织Bcl-2和Bax的影响。方法：采用Lewis肺癌细胞C57BL/6雄性小鼠移植瘤细胞悬液，建立小鼠移植瘤模型。将NF-KB／p65 siRNA接种荷瘤小鼠，应用荧光定量-PCR和Wstern印迹法观察下调NF-kappa;B／p65表达后，肿瘤组织中Bcl-2和BaxmRNA及蛋白表达的变化。结果：经p65siRNA治疗后，p65 siRNA组小鼠肿瘤组织中P65、Bcl-2的mRNA及蛋白表达明显降低而Bax的mRNA及蛋白表达明显升高。差异均有统计学意义（均P ＜0.05）。结论：下调NF-kappa;B/p65的表达，进而抑制Bcl-2表达，处进Bax的表达是使小鼠瘤细胞明显发生凋亡的分子机制之一。说明NF-kappa;B/p65亚基???能在Lewis肺癌细胞凋亡中发挥重要作用，NF-kappa;Bp65因子可能在未来肺癌的靶向治疗中是一个很好的分子靶点。 【关键词】NF-kappa;Bp65；Lewis肺癌；凋亡；siRNA；移植瘤；Bcl-2；Bax. Effect on expression of Bcl-2 and Bax mRNAof nude mouse lung tumour cell xenografts by downregulation NF-kappa;Bp65 Qu Yun1，Zhang Xiaoye2，Wu Rong1 1The Second Department of Oncology，2The Forth Department of Oncology，Shengjing Hospital，China Medical University，39# TengFei Road，Shenyang 110003，LiaoNing Province，China [ABSTRACT]Objectiv：To investigate the effects of expression downregulation of nuclear factor-kappaB（NF-KB）／p65 on the expressions of apoptosis-related genes Bcl-2 and Bax in nude mouse lung tumour cell xenografts .Methods：A nude mouse Lewis lung carcinoma cell xenograft model was established，and the mice were intraperitoneally injected with NF-kappa;B p65 siRNA and sacrificed after 2 weeks of tumour cell injection. Tumour xenografts were harvested for Western blot and quantitative reverse transcription-polymerase chain reaction analyses.Results：Levels of P65 and Bcl-2 mRNA and protein in tumour xenografts were significantly downregulated but Bax was significantly upregulated in p65 siRNA-treated mice compared to the PBS or the NC mice..Conclusion：Knockdown of NF-kappa;B p65 subunit expression significantly downregulated protein P65 and Bcl-2 expression while significantly upregulated protein Bax expression in P65 siRNA group.Targeting NF-kappa;B p65 subunit expression as a potential therapeutic strategy needs further evaluation in treating human lun