中药对衰老糖尿病小鼠模型肾脏病理及部分生化指标的实验研究.docVIP

精品论文 参考文献 中药对衰老糖尿病小鼠模型肾脏病理及部分生化指标的实验研究 右江民族医学院重金属与氟砷毒物研究实验室 广西百色 533000 摘要：目的 探讨中药梅花钻提取液对衰老糖尿病模型肾脏病理及部分生化指标的干预作用.方法 用链佐霉素（STZ）和D-半乳糖及亚硝酸钠溶液联合给小鼠作腹腔注射，建立拟衰老糖尿病模型.然后用中药梅花钻等提取液分别给造模小鼠实验1组、实验2组、实验3组灌服，同时设立不灌药的模型组和正常组。测定造模前、后和灌药前、后各组小鼠血糖及其他生化指标，实验结束，取肾脏作病理检查，取大脑匀浆，测定（ ），对结果作对比分析。结果 肾脏病理检查表明，正常组肾脏：肾小球结构正常，肾小球内无系膜细胞、内皮细胞及脏层上皮细胞增生，系膜区无增宽和沉积物；肾小管上皮无变性、坏死，肾间质无纤维增生和炎性细胞浸润；模型组肾脏：弥漫性肾小球系膜区增宽、系膜基质增多，肾小球内毛细血管内皮细胞轻度增生，毛细血管腔狭窄、关闭，部分肾小球呈节段性硬化、血管袢与球囊壁粘连；近曲小管上皮细胞肿胀、胞浆空泡变性；治疗组肾脏：与模型组基本相似，但较轻。灌药前，正常组血糖明显低于实验组（Plt;0.01），差异有统计学意义；灌药后各组血糖与正常组比较，Plt;0.05，Plt;0.01，差异有统计学意义，治疗前后血糖比较，正常组无变化，治疗2组下降，其他组升高；治疗后，甘油三酯（TG）正常组、治疗2组明显低于其他各组；大脑超氧阴离子自由基（ ）淸除率（%），正常组明显高于其他各组（Plt;0.01），模型组明显低于其他各组（Plt;0.01）。结论 治疗2组lt;广西梅花钻提取液gt;能较好的降低衰老糖尿病模型小鼠血糖和TG，各治疗药均能在一定程度保护肾组织，提高脑组织超氧阴离子自由基（ ）淸除率（%）。 关键词：衰老糖尿病；肾病理；血糖；自由基（ ）； 【Abstract】Objective To investigate interventional effect of kadsura heteroclita（Roxb）craib extract on renal pathology and some biochemical indexes in aging diabetes mouse models.Methods Combination of streptozotocin（STZ），D-galactose and sodium nitrite solution was provided to the mice by intraperitoneal injection.Aging diabetes mouse models were established，and then experimental group one，experimental group two and experimental group three were administered by gavage with kadsura heteroclita（Roxb）craib extract respectively.In the meantime，model group（without being drenched with Chinese plum extract）and normal mouse group were established.Blood glucose and other biochemical indexes of the mice before and after modeling，before and after treatment were respectively tested among groups.After finishing the experiment，the kidneys were removed for pathological examination，and the brain homogenates were taken to test superoxide anion free radicals（ ）.And make comparative analysis on the results.Results Renal pathological examination in the normal mouse group showed that glomerulus structure was normal，without proliferation of glomeruli mesangial cells，endothelial cells and visceral epithelia