匹鲁卡品癫痫模型及戊四氮点燃模型新生鼠行为学和组织学.docVIP

匹鲁卡品癫痫模型及戊四氮点燃模型新生鼠行为学和组织学 【摘要】目的比较匹鲁卡品(毛果芸香碱)点燃新生鼠痫性发作模型及戊四氮点燃模型的行为学特征及组织学改变,为实验模型的选择奠定基础。方法选择SD新生大鼠为研究对象,制作两种不同的新生鼠癫痫模型,观察新生鼠的行为学表现、不同时点Nissl染色等组织学改变。结果两种模型的行为学特征有所不同，匹鲁卡品点燃模型中未见神经元丢失及苔藓出芽现象，戊四氮癫痫持续状态模型中无神经元丢失，但明显出现苔藓纤维出芽现象。结论腹腔注射匹鲁卡品小鼠模型是一种理想的颞叶癫痫动物模型，苔藓纤维出芽可作为判断癫痫持续状态模型是否成功的形态学标准。 癫痫模型；新生鼠；匹鲁卡品；苔藓纤维出芽 志远医学论文网：代写代发医学论文代写医学论文代发医学论文代写医学职称论文医学论文写作期刊发表论文翻译修改 电话QQ：278717711 邮箱：zykkkcn@126.com Comparison study of behavioral and histological changes between pilocarpineinduced epilepsy model and pentylenetetrazole kindling model in the neonatal rat LIU Hongfu1 LI Xiao1 LIU Xiaoran2 et al 1College of Basic Sciences,Binzhou Medical University,Binzhou 256603；2 College of Nursing,Binzhou Medical University 【Abstract】Objective Comparison study of behavioral and histological changes between pilocarpine induced epilepsy model and pentylenetetrazole kindling model in the neonatal rat，to lay the foundation of the experiment model selection.Methods SD neonate rats were selected as the research object.Two different epilepsy models were made to observe the behavioral changes and Nissl staining changes at different time points.Results Two different epilepsy models have their characteristic behavioral changes.No neuron loss and mossy fiber sprouting were found in pilocarpine kindling model.No neuron loss were found with mossy fiber sprouting in pentylenetetrazole statural epilepsy model.Conclusion Pilocarpine induced status epilepticus mouse model is an ideal temporal epilepsy model,and the mossy fiber sprouting is atypical morphological character for evaluating the SE model. 【Key words】epilepsy models,neonatal rat,pilocarpine，mossy fiber sprouting 癫痫动物模型在癫痫的病因学、发病机制、药理学等研究中占重要地位。神经元丢失及苔藓纤维出芽是癫痫产生过程中较常见的组织病理学变化。匹鲁卡品(毛果芸香碱)致癫模型及戊四氮点燃模型均是临床常用的新生鼠癫痫动物模型,两种模型各有不同特点,采用不同的模型可能会有不同的试验结果。目前的试验研究大都选择单一致癫模型，缺少对两种模型癫痫发作过程的对比性研究，本研究分别制作两种癫痫模型，同步观察两种模型不同的行为学特征和组织学改变,并探讨神经元丢失及苔藓纤维出芽在癫痫发病中改变情况[1，2]。 1材料与方法 1.1实验动物与试剂实验动物为烟台绿叶制药公司动物中心提供,生后1 d的SD大鼠60只，随机分为盐水对照组、匹鲁卡品组、戊四氮组,每组20只，分别标记并与母鼠同笼