激动剂作用下α1-肾上腺素受体亚型在HEK293A细胞中的分布变化.pdfVIP

480 Acta Physiologica Sinica, August 25, 2005, 57 (4): 480-485 α - HEK293A 1 ** ** * 100083 α - (α -adrenergic receptor, α -AR)(human embryonic kidney, HEK) 293A 1 1 1 Western blot α -AR (1) α -AR HEK293A 1 1 90% α 1B-AR α 1D -AR α 1A -AR (K d )(2)α1A-AR HEK293A α1B-AR α -AR (3)α -AR (phenylephrine, PE)1 h α - α -AR 1D 1 1A 1B α 1B-AR α 1D-AR PE α 1 -AR HEK293A α 1 - Q 4 6 3 Redistribution of three α -adrenergic receptor subtypes in the stably transfected 1 HEK 293A cells upon agonist stimulation WANG Shu-Yi**, SONG Yao**, XU Ming, HAO Tian-Pao, HAN Qi-De, ZHANG You-Yi* Institute of Vascular Medicine, Peking University Third Hospital and Key Laboratory of Molecular Cardiovascular Sciences, Ministry of Education, Beijing 100083, China Abstract: To investigate the subcellular distribution of three α1-adrenergic receptor subtypes and their internalization and trafficking upon agonist stimulation in human embryonic kidney (HEK) 293A cell line, saturation radioligand binding assay, laser confocal imaging, and Western blot were applied to examine the distribution and changes in localization of three α1-AR subtypes in transfected HEK 293A cells prior to and after treatment with phenylephrine. The results are as follows: (1) The transfection efficiency was over 90% and was equal among three α1-