异养小球藻半连续发酵生产油脂工艺探讨 Explore the Oil Production Process of Chlorella Vulgaris in Semi Continuous Fermentation.pdfVIP
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异养小球藻半连续发酵生产油脂工艺探讨 Explore the Oil Production Process of Chlorella Vulgaris in Semi Continuous Fermentation
食品与发酵科技
FoodandFermentation
Technology
第50卷(第5期)V01.50,No.5
异养小球藻半连续发酵生产油脂工艺探讨
毕生雷1,张成明2,一,李十中2,3刘钺1,杜风光1
(1.河南天冠企业集团有限公司车用生物燃料技术国家重点实验室,河南南阳473000;
2.清华大学核能与新能源技术研究院,北京100084;3.北京市生物燃料工程技术研究中心,北京100084)
摘要:研究了异养小球藻半连续发酵工艺,并对各个生长阶段的细胞数变化、干重变化、单位时间干重变化做了
对比。结论:采用半连续发酵工艺培养异养小球藻时,平均干重增加速率为8.395;g/(L·d),与传统发酵工艺相比,提高
22.38%:平均发酵周期仅216h,与传统发酵工艺相比,发酵周期缩短30.32%;发酵110h后,藻细胞干重质量浓度均
能达到1009/L以上.且油脂含量与传统发酵工艺相差不大。采用半连续发酵工艺,可以提高异养小球藻的整体发酵
效率,有利于规模化应用。
关键词:小球藻:半连续发酵
中图分类号:TQ645 文献标识码:A
theOilProductionProcessofChlorella inSemi
Explore Vulgaris
ContinuousFermentation
BI Yuel,DU
Sheng—leil,ZHANGCheng—min92”,LIShi-zhon92”,QIAOJian-yuanl,LIUFeng-guan91
Motor Henan
Vehicle 473000,China;
(1.State Laboratoryof BiofuelTechnology,HenanTianguanGroupCo.,Ltd,Nanyang
Key
2.InstituteNuclearandNew
of 100084,China;
EnergyTechnology,TsinghuaUniversity,Beoing
Center
Research 100084,China)
3.BeijingEngineering ofBiofuels,Beijing
ofthe Chlorellawas the of
Abstract:Thesemi—continuousfermentation studied,and
process heterotrophic changes
cell foreach were
number,dryweightproduction,anddryweightproductivitygrowthstage investigated.Results:
thesemi-continuousfermentationtocultivatethe Chlorella,thedailydryweight
using process hete
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