干扰3T3-L1脂肪细胞生长激素受体对生长激素诱导的细胞炎症因子表达和分泌的影响.docVIP

干扰3T3-L1脂肪细胞生长激素受体对生长激素诱导的细胞炎症因子表达和分泌的影响 钱慧琳 黄靓 巢汝 周凡 屈顺林 张弛 南华大学心血管病研究所动脉硬化学湖南省重点实验室 X 关注成功！ 加关注后您将方便地在 我的关注中得到本文献的被引频次变化的通知！ 新浪微博 腾讯微博 人人网 开心网 豆瓣网 网易微博 摘????要： 目的:探讨干扰3T3-L1脂肪细胞生长激素受体 (GHR) 对生长激素 (GH) 诱导的脂肪细胞核因子κB (NF-κB) 激活及炎症细胞因子mRNA表达和分泌的影响。方法:采用RNA干扰技术抑制3T3-L1脂肪细胞中GHR的表达;Western blot检测GHR的蛋白表达;双萤光素酶报告基因系统分析GHR对GH激活的3T3-L1脂肪细胞NF-κB转录活性的影响;real-time RT-PCR和ELISA技术检测GHR对GH诱导的3T3-L1脂肪细胞炎症因子mRNA表达和分泌的影响。结果:干扰3T3-L1脂肪细胞GHR的表达能够显著抑制生长激素诱导的细胞NF-κB的激活, 并减少TNF-α、IL-1β和IL-6等炎症细胞因子的mRNA表达和分泌。结论:干扰3T3-L1脂肪细胞GHR可抑制GH诱导的炎症细胞因子表达和分泌。 关键词： 慢性低度炎症; 脂肪细胞; 生长激素; 生长激素受体; 炎症细胞因子; 作者简介：张弛 Tel:0734-8281586;E-mail:zhangchi9966@163.com 收稿日期：2017-05-04 基金：国家自然科学基金资助项目 (NoNo Knockdown of growth hormone receptor prevents growth hormone induced inflammatory cytokine production in 3T3-L1 adipocytes QIAN Hui-lin HUANG Liang CHAO Ru ZHOU Fan QU Shun-lin ZHANG Chi Institute of Cardiovascular Disease, Key Laboratory for Arteriosclerology of Hunan Province, University of South China; Abstract： AIM:To investigate the effect of growth hormone receptor (GHR) knockdown on nuclear factor-κB (NF-κB) activity and inflammatory cytokine production stimulated by growth hormone (GH) in 3 T3-L1 adipocytes.METHODS:The specific siRNA for GHR was transfected into 3 T3-L1 adipocytes to silence GHR expressions.The effects of GH on NF-κB activation and inflammatory cytokine production in 3 T3-L1 adipocytes transfected with siRNA-GHR or siRNA-control were measured by dual-luciferase system analysis, real-time RT-PCR and ELISA.RESULTS:The protein expression of GHR was diminished after transfection with GHR specific siRNA.Dual-luciferase reporter system analysis revealed that GHR knockdown resulted in attenuation of GH-stimulated NF-κB activation in the 3 T3-L1 adipocytes.GHR knockdown ameliorated the GH-induced production of inflammatory cytokines TNF-α, IL-1β, IL-6, MCP-1 and MIP-1α in the 3 T3-L1 adipocytes.CONCLUSION:Knockdown of GHR might be efficacious to prevent GH-induced