Delivery of CatSper2 siRNA into Rat Sperms by Electroporation Repressed Ca2＋ Influx During Sperm Hyperactivation.pdf

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Delivery of CatSper2 siRNA into Rat Sperms by Electroporation Repressed Ca2＋ Influx During Sperm Hyperactivation.pdf

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Delivery of CatSper2 siRNA into Rat Sperms by Electroporation Repressed Ca2＋ Influx During Sperm Hyperactivation

Availableonlineatwvccv．sciencedirect．corn # AgriculturalSciencesinChina 瓣妒ScienceDirect 2011，10(121：1958—1967 December201l DeliveryofCatSper2siRNA intoRatSpermSbyElectroporationRepressed Ca2+lnfluxDuringSperm Hyperactivation ZHANGZhen，ZHOUXuan，LIHui—xia，CUIQun—wei，YUJingandWANGGen．1in CollegeofAnimalScienceandTechnology,NanjingAgriculturalUniversity,Nanjing210095． R ． China Abstract CatSperisauniqueCa channel—likeproteinfamilyexclusively expressedinthetestisandsperm ， andplaysimportant rolesinsperm motility，capacitation，acrosomereactionandsperm—egginteractions ． Herewestudiedthemechanism of regulationofCatSper2一dependentCa外 influx，extracellularandintracellularCa2+onsperm hyperactivatedmotility ． The siRNAduplexesweretransfectedintothespermcellsbyelectroporation(EP)tosilencetheexpressionofCatSper2 ． The resultsfortargeteddisruptionofCatSper2showedthehighestsilenceeffi ciency77 ． 7％ P0．05)，thehyperactivated spermratecalculatedbycomputer—assistedsemenanalysis(CASA)analysisofinterferencedspermwassignificantly lower11．1％ thanthecontrol99．2％