水分胁迫下扶芳藤幼苗保护酶活性和渗透调节物质的变化-植物研究.pdf

23 　4 　　　　　　　　　　　　　　　　　　　　　　 2003 　10 Vol.23　No.4　　　　　　　　　　　BULLETIN OF BOTANICAL RESEARCH Oct., 　2003 1, 2 1 ＊＊ 2 1 3 赵黎芳 　张金政 　张启翔 　石　雷 　鲁韧强 (1., 　100093) (2., 　100083) (3., 　100093) 　　扶芳藤(E uony mus f or t unei (Turcz.)Han .-Mazz)作为北方城市园林的新优攀援藤本 和地被植物越来越受到重视, 新品系的抗 性筛选工作迫在眉睫。 本文采用聚乙二醇(PEG- 6000)模拟水分胁迫的方法, 测定其生理指标, 研究水分胁迫对扶芳藤不同品系幼苗叶片保护酶 活性和渗透调节物质的影响, 旨在摸索出这些生理指标与植物抗旱性的关系。研究结果表明, 随着模拟干旱时间的延长, 扶芳藤幼苗叶片中相对含水量(RWC )逐步下降, 保护酶活性上升, 脯氨酸和可溶性糖含量急剧上升, 并且抗旱性强的品系保护酶活性和渗透调节物质含量较高。 　水分胁迫;扶芳藤;保护酶;渗透调节 EFFECT OF WATER STRESS ON ACTIVITY OF CELL DEFENSE ENZYMES AND OSMOTIC ADJUSTMENT IN E UON YM US FOR T UNEI YOUNG PLANT 1, 2 1 ＊ 2 1 3 ZHAO Li-Fang 　ZHANG Jin-Zheng 　ZHANG Qi-Xiang 　SHI Lei 　LU Ren-Qiang (1.Institute of Botany, the Chinese Aca emy of Sciences, Beijing　100093) (2.Beijing Forest University, Beijing　100083) (3.Institute of Forestry an Pomology, Beijing Aca emy of Agriculture an Forestry, Beijing 　100093) Abstract　People are attaching more importance to E uony mus f or tunei, w hich is a new ly excel- lent climbing vine.However, the selecting experiment is staring in the face.In this stu y, E uony - mus f or tunei young plants w ere treate w ith the osmotic stress solution PEG-6000.The relation between the plant physiology in exes an plant rought tolerance w ere etermine .The results showe that , along with time, relative w ater content (RWC)escen e an efense enzymes activi- ties ascen e .Proline an soluble sugar content hoike .The variety of high