鸡组织中链霉素残留检测方法的研究.PDFVIP

吉林农业大学学报 2005,27(3) :339~ 343 Journal of Jilin Agricultural University * ** 董晓庆, 程培英 , 陈绍辉, 曲桂娟 (吉林农业大学动物科技学院, 长春 130118) : 采用柱前衍生化紫外检测器高效液相色 法(HPLC) 检测鸡组织中链霉素的残留量, 优化了样品的 前处理方法和高效液相色 条件( 包括流动相的组成和流速) , 同时优化了前处理过程中洗脱液和提取液的用 量结果表明:流动相为 V( 乙腈)B V( 醋酸盐缓冲液pH= 31 3)B V( 水)= 10B10B80, 流速013mLPmin,柱温35e , 检测波长252 nm,进样量20LL 为最佳检测条件采用沉淀剂(5% 5mL 三氯醋酸) 沉淀蛋白质, 洗脱剂( 10 mL 甲醇) 洗脱,在3 种不同鸡组织中添加200, 100,50 LgPmL 3 种标准溶液, 测定其回收率分别为75%~ 90%, 70%~ 86%,64%~ 75%,变异系数均[ 10% 同一试样设10 个平行样, 其变异系数为5172%, 说明该方法的 重现性较好 : 链霉素; 高效液相色 法; 紫外检测; 柱前衍生化 : S816173 : A : 1000-5684( 2005)03-0339-05 Study on the Method of Detecting Streptomycin Residues in Chicken Tissues D NG Xiao-qing, CHENG Pei-ying, CHEN Shao-hui, QU Gui-juan ( Colleg e of A nimal Science and Technology , Jilin Agricultural Univ ers ity , Changchun 130118, China) Abstract: HPLC with pre-column derivation and ultraviolet detection were used to detect streptomycin residues in chicken tissues1 Detection conditions of HPLC and disposal method of sample including the proportion and speed of mobile phase, dosage of solution and elution were optimized1 The results showed that therewas abest detection condition when the proportion and speed of mobile phasewere acetonitrile, acetic acid buffer solution and water ( 10B10B80) , speed was 013 mLPmin,temperature of column was 35e ,wavelength was 252 nm,and quantity of injection was 20 LL1 The rate recoveries of three different tissues and different addition concentrationswere determined by using precipitant (5% ,5mL) and eluant ( 10 mL) 1 The rates of recoveries were 75%~ 90%, 70%~ 86% and 64%~ 75% respectively when